Rabbit NOTUM Polyclonal Antibody | anti-NOTUM antibody

NOTUM Antibody

Cat. Num. AAA151515

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NOTUM Antibody is affinity chromatography purified via peptide column.
• Synonyms: NOTUM; Polyclonal Antibody; NOTUM Antibody; Protein notum homolog; notum pectinacetylesterase homolog (Drosophila); anti-NOTUM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NOTUM Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 496

• Applicable Applications for anti-NOTUM antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NOTUM antibody can be used for detection of NOTUM by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NOTUM antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human NOTUM.
• Buffer: NOTUM Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NOTUM antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NOTUM in human kidney tissue lysate with NOTUM antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NOTUM in human brain tissue with NOTUM antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NOTUM in Mouse Kidney cells with NOTUM antibody at 20 μg/mL.)

Related Product Information for anti-NOTUM antibody

NOTUM Antibody: In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by beta-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 194394139
• NCBI GeneID: 147111
• NCBI Accession #: NP_848588
• NCBI GenBank Nucleotide #: NM_178493.5
• UniProt Accession #: Q6P988; Q8N410; Q8NI82
• Molecular Weight: 55,699 Da
• NCBI Official Full Name: protein notum homolog
• NCBI Official Synonym Full Names: notum pectinacetylesterase homolog (Drosophila)
• NCBI Official Symbol: NOTUM
• NCBI Protein Information: protein notum homolog
• UniProt Protein Name: Protein notum homolog
• Protein Family: Palmitoleoyl-protein carboxylesterase
• UniProt Gene Name: NOTUM
• UniProt Entry Name: NOTUM_HUMAN

Uniprot Description

NOTUM: May deacetylate GlcNAc residues on cell surface glycans (Potential). Belongs to the pectinacetylesterase family.

Protein type: Secreted, signal peptide; Hydrolase; Secreted; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular region

Molecular Function: hydrolase activity

Biological Process: metabolic process