Testing Data
Goat GFP Polyclonal Antibody | anti-GFP antibody
Anti-GFP Antibody
Reactivity
GFP and its varients.
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoe
Synonyms
GFP; Polyclonal Antibody; Anti-GFP Antibody; anti-GFP antibody
Host
Goat
Reactivity
GFP and its varients.
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognize GFP and its variants.
Purity/Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoe
Form/Format
Liquid
Concentration
100ug/100ul (varies by lot)
Sequence Length
238
Applicable Applications for anti-GFP antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Microscopy
Application Notes
ELISA: 1:40,000
WESTERN BLOT: 1:400 - 1:2,000
IMMUNOHISTOCHEMISTRY: 1:200 - 1:1,000
IF MICROSCOPY: 1:1,000
IP: 1:200
WESTERN BLOT: 1:400 - 1:2,000
IMMUNOHISTOCHEMISTRY: 1:200 - 1:1,000
IF MICROSCOPY: 1:1,000
IP: 1:200
Storage Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Immunogen
The anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
References
Sutton, LM et al. "Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease." Hum Mol Genet 22 (3):452-465 (2013). DOI: 10.1093/hmg/dds441. Application: Immunoprecipitation.
Luchniak, A et al. "Structure-function analysis of yeast." Methods Cell Biol 115:355-374 (2013). DOI: 10.1016/B978-0-12-407757-7.00022-0. Application: SDS-PAGE.
Luchniak, A et al. "Structure-function analysis of yeast." Methods Cell Biol 115:355-374 (2013). DOI: 10.1016/B978-0-12-407757-7.00022-0. Application: SDS-PAGE.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GFP (Catalog #AAA417047) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-GFP Antibody reacts with GFP and its varients. and may cross-react with other species as described in the data sheet. AAA Biotech's GFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Microscopy. ELISA: 1:40,000 WESTERN BLOT: 1:400 - 1:2,000 IMMUNOHISTOCHEMISTRY: 1:200 - 1:1,000 IF MICROSCOPY: 1:1,000 IP: 1:200. Researchers should empirically determine the suitability of the GFP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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