Rabbit anti-Human, Mouse Nitrotyrosine Polyclonal Antibody | anti-nTyr antibody

Nitrotyrosine (nTyr) (PE)

Cat. Num. AAA6490098

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Nitrotyrosine; Polyclonal Antibody; Nitrotyrosine (nTyr) (PE); anti-nTyr antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes nitrated tyrosine residues. Species Crossreactivity: human, mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-nTyr antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:50-1:500 mouse brain (animal treated with Zymosan) with pretreatment with citrate buffer, pH 6.0.; WB: 1:100-1:1000 when tested against nitrated BSA and nitrated ovalbumin.; Applications are based on unconjugated antibody.
• Immunogen: Nitrated KLH
• Conjugate: PE
• Positive Control: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10ul of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Nytrotyrosine staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. A 1:500 dilution of MBS612511 was used followed by HRP/DAB staining. Immunoreactivity is seen in the hippocampus.)

Immunohistochemistry (IHC)

(Negative control staining of normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, Negative Control Reagent, IHC Select detection withMBS612511. No signal detected.)

Immunohistochemistry (IHC)

(Nitrotyrosine staining of polyclonal normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, pAb at 1:500 overnight incubation, IHC Select detection withMBS612511. Immunoreactivity is seen in the neurons)

Immunohistochemistry (IHC)

(Negative control staining of normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, Negative Control Reagent, IHC Select detection with MBS612511 No signal detected.)

Product Categories/Family for anti-nTyr antibody

Antibodies; Nitrotyrosine