Rabbit anti-Human NIK Polyclonal Antibody | anti-NIK antibody

NIK (CT)

Cat. Num. AAA395687

• Gene Names: MAP3K14; HS; NIK; HSNIK; FTDCR1B
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: NIK; Polyclonal Antibody; NIK (CT); NF Kappa B inducing kinase; Serine/threonine-protein kinase NIK; HsNIK; Mitogen activated protein kinase kinase kinase 14 (M3K14); anti-NIK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Provided as solution in phosphate buffered saline with 0.02% sodium azide
• Sequence Length: 947

• Applicable Applications for anti-NIK antibody: Western Blot (WB)
• Immunogen: Rabbit polyclonal NIK antibody was raised against a peptide corresponding to amino acids 931 to 947 of human NIK.
• Positive Control: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. Located in the cytoplasm.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-NIK antibody

Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF alpha LPS and mitogens. A serine/threonine protein kinase which mediates NFkappa;B activation by IL-1, TNF alpha; and CD95 was identified recently and designated NIK (for NF kappa;B inducing kinase). NIK is an activator of I kappa B kinase alpha; and beta; (IKK alpha; and IKK beta;) (2-5). Therefore, NIK is a key molecule in the NF kappa;B signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.

Product Categories/Family for anti-NIK antibody

Polyclonal Antibody; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 115298645
• NCBI GeneID: 9020
• NCBI Accession #: NP_003945.2
• NCBI GenBank Nucleotide #: NM_003954.3
• UniProt Accession #: Q99558; Q8IYN1; A8K2D8; D3DX67
• Molecular Weight: 104 kD
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 14
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 14
• NCBI Official Symbol: MAP3K14
• NCBI Official Synonym Symbols: HS; NIK; HSNIK; FTDCR1B
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; serine/threonine protein-kinase; serine/threonine-protein kinase NIK
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 14
• Protein Family: Nik-related protein kinase
• UniProt Gene Name: MAP3K14
• UniProt Synonym Gene Names: NIK; HsNIK
• UniProt Entry Name: M3K14_HUMAN

NCBI Description

This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts with TRAF2, TRAF5, TRAF6, IKKA and NFKB2/P100

By similarity. Interacts with TRAF3 and PELI3. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with GRB10. Interacts with ZFP91. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.15

Subcellular location: Cytoplasm.

Tissue specificity: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. Ref.1

Post-translational modification: Autophosphorylated. Phosphorylation at Thr-559 is required to activates its kinase activity and 'Lys-63'-linked polyubiquitination. Phosphorylated by CHUK/IKKA leading to MAP3K14 destabilization. Ref.11 Ref.15Ubiquitinated. Undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. 'Lys-48'-linked polyubiquitination leads to its degradation by the proteasome, while 'Lys-63'-linked polyubiquitination stabilizes and activates it. Ref.8 Ref.15

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.