Rabbit anti-Human NIK Polyclonal Antibody | anti-MAP3K14 antibody

NIK Antibody

Cat. Num. AAA150367

• Gene Names: MAP3K14; HS; NIK; HSNIK; FTDCR1B
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: NIK Antibody is Antibody is DEAE purified.
• Synonyms: NIK; Polyclonal Antibody; NIK Antibody; HS; HSNIK; FTDCR1B; Mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; HsNIK; mitogen-activated protein kinase kinase kinase 14; anti-MAP3K14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NIK Antibody is Antibody is DEAE purified.
• Form/Format: Liquid
• Sequence Length: 947

• Applicable Applications for anti-MAP3K14 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: NIK antibody can be used for detection of NIK by Western blot.
• Conjugate: Unconjugated
• Immunogen: NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK.
• Buffer: NIK Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NIK antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Immunocytochemistry (IHC)

(Immunocytochemistry of NIK in Hek293 cells with NIK antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NIK in Hek293 cells with NIK antibody at 20 μg/mL.Green: NIK Antibody (1129)Blue: DAPI staining)

Related Product Information for anti-MAP3K14 antibody

NIK Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.

NCBI and Uniprot Product Information

• NCBI GI #: 92090612
• NCBI GeneID: 9020
• UniProt Accession #: Q99558; Q8IYN1; A8K2D8; D3DX67
• Molecular Weight: 104,042 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 14
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 14
• NCBI Official Symbol: MAP3K14
• NCBI Official Synonym Symbols: HS; NIK; HSNIK; FTDCR1B
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 14; NF-kappa-beta-inducing kinase; serine/threonine-protein kinase NIK
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 14
• Protein Family: Nik-related protein kinase
• UniProt Gene Name: MAP3K14
• UniProt Synonym Gene Names: NIK; HsNIK
• UniProt Entry Name: M3K14_HUMAN

NCBI Description

This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Nik: a protein kinase of the STE group of kinases. Binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.

Protein type: EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, STE; STE group; STE-Unique family

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytosol

Molecular Function: NF-kappaB-inducing kinase activity; MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; T cell costimulation; MAPKKK cascade; immune response; protein amino acid phosphorylation