Rabbit NIK Polyclonal Antibody | anti-NIK antibody

NIK Antibody

Cat. Num. AAA840977

• Gene Names: MAP3K14; HS; NIK; HSNIK; FTDCR1B
• Reactivity: Human, Mouse, Rat, Horse, Dog
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: NIK; Polyclonal Antibody; NIK Antibody; MAP3K14; hsNIK; FTDCR1B; HSNIK; EC 2.7.11.25; anti-NIK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Horse, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 0.5 mg/ml affinity purified rabbit anti-NIK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 5 mM EDTA and 0.03% proclin; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 947

• Applicable Applications for anti-NIK antibody: Western Blot (WB)
• Application Notes: The antibody can be used for Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
• Immunogen: Synthetic peptide surrounding amino acid 927of human NIK
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: Store at -20°C; For long term storage , aliquot at -70°C.; Avoid repeated freeze/defrost cycles

Testing Data

(MBS840977)

Related Product Information for anti-NIK antibody

Background: NIK (NF-kB-inducing kinase) is a member of the MAP kinase kinase kinase family that binds TRAF2 and stimulates NF-kB activity. NIK was initially isolated from a human B cell cDNA library and contains 795 amino acids with an apparent molecular weight of slightly more than 97 kDa on SDS gel. NIK is a serine/threonine kinase and its kinase activity contributes to IkB phosphorylation. The carboxyl terminal segment of NIK binds TRAF2. A mutant NIK with intact carboxyl terminus but without the two lysine residues at its catalytic domain serves as a dominant-negative inhibitor for NF-kB activation. NIK also interacts with TRAF6 and mediates IL-1-induced NF-kB activation.

NCBI and Uniprot Product Information

• NCBI GI #: 115298645
• NCBI GeneID: 9020
• NCBI Accession #: NP_003945.2
• NCBI GenBank Nucleotide #: NM_003954.4
• UniProt Accession #: Q99558; Q8IYN1; A8K2D8; D3DX67
• Molecular Weight: 104,042 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 14
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 14
• NCBI Official Symbol: MAP3K14
• NCBI Official Synonym Symbols: HS; NIK; HSNIK; FTDCR1B
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 14
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 14
• Protein Family: Nik-related protein kinase
• UniProt Gene Name: MAP3K14
• UniProt Synonym Gene Names: NIK; HsNIK
• UniProt Entry Name: M3K14_HUMAN

NCBI Description

This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

Nik: a protein kinase of the STE group of kinases. Binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE-Unique family

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytosol

Molecular Function: MAP kinase kinase activity; NF-kappaB-inducing kinase activity; protein binding; MAP kinase kinase kinase activity; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; T cell costimulation; MAPKKK cascade; immune response; protein amino acid phosphorylation