Rabbit NFYA Monoclonal Antibody | anti-NFYA antibody

NFYA Rabbit mAb

Cat. Num. AAA4752910

• Gene Names: NFYA; HAP2; CBF-A; CBF-B; NF-YA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: NFYA; Monoclonal Antibody; NFYA Rabbit mAb; Nuclear transcription factor Y subunit alpha; HAP2; CBF-A; CBF-B; NF-YA; anti-NFYA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R01-5G1
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-NFYA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000-1:5000; ICC/IF: 1:20-1:50; FC/FACS: 1:20; IP: 1:20-1:50
• Immunogen: A synthetic peptide of human NFYA
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of NFYA in C6,3T3,Hela cell lysates using NFYA Rabbit mAb(1:1000 diluted). Predicted band size:37kDa. Observed band size:37kDa.)

Related Product Information for anti-NFYA antibody

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]
Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.

NCBI and Uniprot Product Information

• NCBI GI #: 4505389
• NCBI GeneID: 4800
• NCBI Accession #: NP_002496.1
• NCBI GenBank Nucleotide #: NM_002505.4
• UniProt Accession #: P23511; Q8IXU0
• Molecular Weight: 36,877 Da
• NCBI Official Full Name: nuclear transcription factor Y subunit alpha isoform 1
• NCBI Official Synonym Full Names: nuclear transcription factor Y, alpha
• NCBI Official Symbol: NFYA
• NCBI Official Synonym Symbols: HAP2; CBF-A; CBF-B; NF-YA
• NCBI Protein Information: nuclear transcription factor Y subunit alpha; HAP2 CCAAT-binding protein; Transcription factor NF-Y, A subunit; CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; nuclear transcription factor Y subunit A; CCAAT-binding transcr
• UniProt Protein Name: Nuclear transcription factor Y subunit alpha
• Protein Family: Nuclear transcription factor
• UniProt Gene Name: NFYA
• UniProt Synonym Gene Names: NF-YA
• UniProt Entry Name: NFYA_HUMAN

NCBI Description

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

NFYA: stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. Involved in cisplatin resistance. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; CCAAT-binding factor complex; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; rhythmic process; positive regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process