EPHA1 active protein

EphA1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123006

• Gene Names: Epha1; Eph; Esk; AL033318; 5730453L17Rik
• Purity: >97% as determined by SDS-PAGE
• Synonyms: EphA1; EphA1 Protein; Mouse; Recombinant (His Tag); Mouse EphA1/EPH receptor A1 Protein (His Tag); 5730453L17Rik Protein; AL033318 Protein; Eph Protein; Esk Protein; EPH receptor A1; EPHA1 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu548

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse EPHA1 (Q60750)(Met 1-Glu 548) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10ug/ml (100uL/well) can bind mouse EFNA1-Fc. The EC50 of mouse EFNA1-Fc is 21.3-49.8ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 ug/ml (100 ul/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.)

Related Product Information for EPHA1 active protein

Background: EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.

Product Categories/Family for EPHA1 active protein

Actin Dynamics Signaling Pathway

References

Flanagan JG, et al. (1998) THE EPHRINS AND EPH RECEPTORS IN NEURAL DEVELOPMENT. Annual Review of Neuroscience. 21: 309-45.Wilkinson DG (2000) Eph receptors and ephrins: Regulators of guidance and assembly. International Review of Cytology. 196: 177-244.Zhou R. (1998) The Eph family receptors and ligands. Pharmacol. 77 (3): 151-81.

NCBI and Uniprot Product Information

• NCBI GI #: 31980651
• NCBI GeneID: 13835
• NCBI Accession #: NP_076069.2
• NCBI GenBank Nucleotide #: NM_023580.4
• UniProt Accession #: Q60750; Q8CED9; Q9ESJ2
• Molecular Weight: 108,578 Da
• NCBI Official Full Name: ephrin type-A receptor 1
• NCBI Official Synonym Full Names: Eph receptor A1
• NCBI Official Symbol: Epha1
• NCBI Official Synonym Symbols: Eph; Esk; AL033318; 5730453L17Rik
• NCBI Protein Information: ephrin type-A receptor 1; mEpha1; embryonic stem cell kinase; tyrosine-protein kinase receptor ESK
• UniProt Protein Name: Ephrin type-A receptor 1
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha1
• UniProt Synonym Gene Names: Esk; mEpha1
• UniProt Entry Name: EPHA1_MOUSE

Uniprot Description

EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; Kinase, protein; EC 2.7.10.1; TK group; Eph family

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; transmembrane-ephrin receptor activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; nucleotide binding; kinase activity; transmembrane receptor protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of angiogenesis; cell surface receptor linked signal transduction; positive regulation of cell proliferation; positive regulation of stress fiber formation; ephrin receptor signaling pathway; negative regulation of protein kinase activity; angiogenesis; positive regulation of cell-matrix adhesion; cell adhesion; phosphorylation; negative regulation of cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration