Western Blot (WB)
(Western Blot: HeLa nuclear extract (20 ug per lane) probed with the Histone H1.5 phospho Ser17 antibody (pAb) at a dilution of 1:1,000.)
Rabbit Histone H1.5S17ph Polyclonal Antibody | anti-H1.5S17ph antibody
Histone H1.5S17ph antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Dot Blot, Western Blot
Purity
Affinity Purified
Synonyms
Histone H1.5S17ph; Polyclonal Antibody; Histone H1.5S17ph antibody (pAb); anti-H1.5S17ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H1.5S17ph antibody
Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot.
Conjugation
Phosphorylated
Immunogen
This Histone H1.5 phospho Ser17 antibody was raised against a peptide containing phospho Ser17 of human histone H1.5.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Western Blot: HeLa nuclear extract (20 ug per lane) probed with the Histone H1.5 phospho Ser17 antibody (pAb) at a dilution of 1:1,000.)
Western Blot (WB)
(Western Blot: HeLa nuclear extract (20 ug per lane) probed with the Histone H1.5 phospho Ser17 antibody (pAb) at a dilution of 1:1,000.)
Dot Blot (DB)
(Specificity Data: Dot blot analysis was used to confirm the specificity of the Histone H1.5 phospho Ser17 antibody. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: phospho Ser17 peptide. Lane 2: unmodified Ser17 peptide. Lane 3: phospho Thr17 histone H1.4 peptide.)
Dot Blot (DB)
(Specificity Data: Dot blot analysis was used to confirm the specificity of the Histone H1.5 phospho Ser17 antibody. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: phospho Ser17 peptide. Lane 2: unmodified Ser17 peptide. Lane 3: phospho Thr17 histone H1.4 peptide.)
Related Product Information for anti-H1.5S17ph antibody
Short Description: Our Histone H1.5S17ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3.
Background: The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points; it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; they play a major role in regulating gene expression. Histone H1.5 is an H1 isotype expressed in somatic cells and is heavily phosphorylated during mitosis. Phosphorylation of H1.5 at serine 17 appears in prometaphase and disappears in telophase. The hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin is maximally condensed. Phosphorylation of H1.5 at serine 17 can be catalyzed by GSK-3.
Product Categories/Family for anti-H1.5S17ph antibody
NCBI and Uniprot Product Information
Molecular Weight
36kDa
Similar Products
Product Notes
The H1.5S17ph (Catalog #AAA388762) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H1.5S17ph antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H1.5S17ph can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western Blot. Researchers should empirically determine the suitability of the H1.5S17ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H1.5S17ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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