Western Blot (WB)
(Histone H3.3 phospho Ser31 antibody tested by Western blot. Detection of Histone H3.3 by Western blot. The analysis was performed using 20 ug of untreated (lane 1) or colcemid treated (lane 2) HeLa acid extract with a 1:1000 dilution of Histone H3.3 phospho Ser31 antibody.)
Rabbit Histone H3.3S31ph Polyclonal Antibody | anti-H3.3S31ph antibody
Histone H3.3S31ph antibody (pAb)
Reactivity
Human, Wide Range Predicted
Applications
Dot Blot, Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
Histone H3.3S31ph; Polyclonal Antibody; Histone H3.3S31ph antibody (pAb); anti-H3.3S31ph antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-H3.3S31ph antibody
Dot Blot (DB), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500 dilution
WB: 1:500 dilution
Conjugation
Phosphorylated
Immunogen
This Histone H3.3 phospho Ser31 antibody was raised against a peptide containing phospho Ser31 of Histone H3.3.
Abs Residue
Ser
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Histone H3.3 phospho Ser31 antibody tested by Western blot. Detection of Histone H3.3 by Western blot. The analysis was performed using 20 ug of untreated (lane 1) or colcemid treated (lane 2) HeLa acid extract with a 1:1000 dilution of Histone H3.3 phospho Ser31 antibody.)
Western Blot (WB)
(Histone H3.3 phospho Ser31 antibody tested by Western blot. Detection of Histone H3.3 by Western blot. The analysis was performed using 20 ug of untreated (lane 1) or colcemid treated (lane 2) HeLa acid extract with a 1:1000 dilution of Histone H3.3 phospho Ser31 antibody.)
Dot Blot (DB)
(Histone H3.3 phospho Ser31 antibody tested by Dot blot. Dot blot analysis was used to confirm the specificity of Histone H3.3 phospho Ser31 antibody. Peptides corresponding to histone H3.3 and regions around major sites of histone H3 phosphorylation (serine 10 and serine 28) were spotted onto PVDF and probed with 39637 at a dilution of 1:10,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 31 of H3.3. Lane 2: unmodified serine 31 peptide. Lane 3: peptide phosphorylated at serine 10 of H3. Lane 4: peptide phosphorylated at serine 28 of H3.)
Dot Blot (DB)
(Histone H3.3 phospho Ser31 antibody tested by Dot blot. Dot blot analysis was used to confirm the specificity of Histone H3.3 phospho Ser31 antibody. Peptides corresponding to histone H3.3 and regions around major sites of histone H3 phosphorylation (serine 10 and serine 28) were spotted onto PVDF and probed with 39637 at a dilution of 1:10,000. The amount of peptide (in picomoles) spotted is indicated next to each row. Lane 1: peptide phosphorylated at serine 31 of H3.3. Lane 2: unmodified serine 31 peptide. Lane 3: peptide phosphorylated at serine 10 of H3. Lane 4: peptide phosphorylated at serine 28 of H3.)
Related Product Information for anti-H3.3S31ph antibody
Short Description: Our Histone H3.3S31ph antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot, Immunofluorescence and Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3.3 (H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Background: Histone H3.3 (H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes.
Product Categories/Family for anti-H3.3S31ph antibody
References
Hake et al. (2005) PNAS 102: 6344-6349.
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3.3S31ph (Catalog #AAA388522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3.3S31ph antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3.3S31ph can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Immunofluorescence (IF), Western Blot (WB). Applications Validated: WB: 1:500 dilution. Researchers should empirically determine the suitability of the H3.3S31ph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3.3S31ph, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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