Rabbit anti-Human NFkB, p65, NLS Specific Polyclonal Antibody
NFkB, p65, NLS Specific (NF-kB, Nuclear Factor kappa B, RelA)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
NFkB; p65; NLS Specific; Polyclonal Antibody; NLS Specific (NF-kB; Nuclear Factor kappa B; RelA); Anti -NFkB; anti-NFkB; NLS Specific antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes the nuclear localization sequence (NLS) NLS of human p65. The epitope overlaps the NLS of the p65 subunit of the NFkB heterodimer.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2 0.1% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.
Applicable Applications for anti-NFkB, p65, NLS Specific antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays.
Dilution: Western Blot: 1:1000
Immunohistochemistry: 1:100
Immunofluorescence microscopy: 1:100
Super shift: 0.5-1ul/assay.
Dilution: Western Blot: 1:1000
Immunohistochemistry: 1:100
Immunofluorescence microscopy: 1:100
Super shift: 0.5-1ul/assay.
Immunogen
A synthetic peptide, RIEEKRKRTYET, corresponding to the NLS of the human NFkB p65 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-NFkB, p65, NLS Specific antibody
NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as aheterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding IkB-a binding masks the NFkB nuclear localisation signal (NLS).
Product Categories/Family for anti-NFkB, p65, NLS Specific antibody
Similar Products
Product Notes
The NFkB, p65, NLS Specific (Catalog #AAA615742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFkB, p65, NLS Specific (NF-kB, Nuclear Factor kappa B, RelA) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB, p65, NLS Specific can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA). Suitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays. Dilution: Western Blot: 1:1000 Immunohistochemistry: 1:100 Immunofluorescence microscopy: 1:100 Super shift: 0.5-1ul/assay. Researchers should empirically determine the suitability of the NFkB, p65, NLS Specific for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB, p65, NLS Specific, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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