Rabbit anti-Human AP2S1 Polyclonal Antibody | anti-AP2S1 antibody

AP2S1, ID (AP2S1, AP17, CLAPS2, AP-2 complex subunit sigma, Adapter-related protein complex 2 sigma subunit, Adaptor protein complex AP-2 subunit sigma, Clathrin assembly protein 2 small chain, Clathrin coat assembly protein AP17, Clathrin coat-associated

Cat. Num. AAA6274096

• Gene Names: AP2S1; AP17; HHC3; CLAPS2; AP17-DELTA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP2S1; Polyclonal Antibody; ID (AP2S1; AP17; CLAPS2; AP-2 complex subunit sigma; Adapter-related protein complex 2 sigma subunit; Adaptor protein complex AP-2 subunit sigma; Clathrin assembly protein 2 small chain; Clathrin coat assembly protein AP17; Clathrin coat-associated; Clathrin coat-associated protein AP17; HA2 17kD subunit; Plasma membrane adaptor AP-2 17kD protein; Sigma2-adaptin; anti-AP2S1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AP2S1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AP2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-64 amino acids from the Central region of human AP2S1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AP2S1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AP2S1 antibody detected the AP2S1 protein (arrow).)

Related Product Information for anti-AP2S1 antibody

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts.

Product Categories/Family for anti-AP2S1 antibody

Antibodies; Clathrin

References

Stove, V., et al. J. Virol. 79(17):11422-11433(2005). Lu, W., et al. Neuron 47(3):407-421(2005). Collins, B.M., et al. Cell 109(4):523-535(2002). Yano, H., et al. J. Neurosci. 21 (3), RC125 (2001): Pearse, B.M., et al. Curr. Opin. Struct. Biol. 10(2):220-228(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 70906430
• NCBI GeneID: 1175
• NCBI Accession #: NP_004060.2; NP_067586.1
• NCBI GenBank Nucleotide #: NM_004069.3
• UniProt Accession #: P53680; O75977; Q6PK67; B2R4Z4
• Molecular Weight: 17,018 Da
• NCBI Official Full Name: AP-2 complex subunit sigma isoform AP17
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, sigma 1 subunit
• NCBI Official Symbol: AP2S1
• NCBI Official Synonym Symbols: AP17; HHC3; CLAPS2; AP17-DELTA
• NCBI Protein Information: AP-2 complex subunit sigma; sigma2-adaptin; HA2 17 kDa subunit; clathrin coat assembly protein AP17; clathrin coat-associated protein AP17; clathrin assembly protein 2 small chain; adaptor protein complex AP-2 subunit sigma; plasma membrane adaptor AP-2 1
• UniProt Protein Name: AP-2 complex subunit sigma
• Protein Family: AP-2 complex
• UniProt Gene Name: AP2S1
• UniProt Synonym Gene Names: AP17; CLAPS2
• UniProt Entry Name: AP2S1_HUMAN

NCBI Description

One of two major clathrin-associated adaptor complexes, AP-2, is a heterotetramer which is associated with the plasma membrane. This complex is composed of two large chains, a medium chain, and a small chain. This gene encodes the small chain of this complex. Alternative splicing has been observed in this gene and results in two known transcripts. [provided by RefSeq, Jul 2008]

Uniprot Description

AP2S1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes small subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: plasma membrane; AP-2 adaptor complex; cytosol

Molecular Function: transporter activity; protein transporter activity

Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; intracellular protein transport; axon guidance; synaptic transmission; clathrin cage assembly; viral reproduction; nerve growth factor receptor signaling pathway; regulation of endocytosis; ephrin receptor signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class II; regulation of defense response to virus by virus

Disease: Hypocalciuric Hypercalcemia, Familial, Type Iii