Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) RTU ELISA Kit | ADCYAP1 rtu elisa kit

Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) ELISA Kit

Cat. Num. AAA457399

• Gene Names: ADCYAP1; PACAP
• Reactivity: Human
• Synonyms: Adenylate Cyclase Activating Polypeptide 1; Pituitary (ADCYAP1); Human Adenylate Cyclase Activating Polypeptide 1; Pituitary (ADCYAP1) ELISA Kit; PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide; ADCYAP1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 176

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 7.81-500pg/mL
• Sensitivity: 2.3pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADCYAP1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADCYAP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADCYAP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADCYAP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADCYAP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 153266792
• NCBI GeneID: 116
• NCBI Accession #: NP_001093203.1
• NCBI GenBank Nucleotide #: NM_001099733.1
• UniProt Accession #: P18509; Q52LQ0; B2R7N4
• Molecular Weight: 18,835 Da
• NCBI Official Full Name: pituitary adenylate cyclase-activating polypeptide preproprotein
• NCBI Official Synonym Full Names: adenylate cyclase activating polypeptide 1
• NCBI Official Symbol: ADCYAP1
• NCBI Official Synonym Symbols: PACAP
• NCBI Protein Information: pituitary adenylate cyclase-activating polypeptide
• UniProt Protein Name: Pituitary adenylate cyclase-activating polypeptide
• Protein Family: Glucagon family neuropeptides
• UniProt Gene Name: ADCYAP1
• UniProt Synonym Gene Names: PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38

NCBI Description

This gene encodes a secreted proprotein that is further processed into multiple mature peptides. These peptides stimulate adenylate cyclase and increase cyclic adenosine monophosphate (cAMP) levels, resulting in the transcriptional activation of target genes. The products of this gene are key mediators of neuroendocrine stress responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

Uniprot Description

Binding to its receptor activates G proteins and stimulates adenylate cyclase in pituitary cells. Promotes neuron projection development through the RAPGEF2/Rap1/B-Raf/ERK pathway. In chromaffin cells, induces long-lasting increase of intracellular calcium concentrations and neuroendocrine secretion (). Involved in the control of glucose homeostasis, induces insulin secretion by pancreatic beta cells ().