Western Blot (WB)
(Western blot analysis of NFkB expression in Jurkat cell lysate.)
Rabbit NFkB p65 Polyclonal Antibody | anti-NFkB antibody
NFkB p65 Antibody
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
NFkB p65; Polyclonal Antibody; NFkB p65 Antibody; Rabbit Polyclonal NFkB p65 Antibody; Polyclonal NFkB p65 antibody; anti-NFkB p65 antibody; NFKB3 antibody; NFkB p 65; NFkB p-65 antibody; NFkB p 65 antibody; RELA antibody; NFkB p-65; MGC131774 antibody; anti-NFkB antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer, pH 7.2, containing 25% Glycerol, 0.5% BSA, 0.01% Thimerosal
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-NFkB antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ICC: 10-20 ug/ml
WB: 0.5-4 ug/ml
WB: 0.5-4 ug/ml
Immunogen
NFkB p65 antibody was raised in Rabbit using synthetic peptide surrounding amino acid 274 of mouse NFkB p65 as the immunogen.
Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of NFkB expression in Jurkat cell lysate.)
Western Blot (WB)
(Western blot analysis of NFkB expression in Jurkat cell lysate.)
Related Product Information for anti-NFkB antibody
NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit and a 65 kDa transactivation subunit. Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide, pro-inflammatory cytokines, and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
Product Categories/Family for anti-NFkB antibody
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Product Notes
The NFkB (Catalog #AAA5311137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NFkB p65 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). ICC: 10-20 ug/ml WB: 0.5-4 ug/ml. Researchers should empirically determine the suitability of the NFkB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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