Rabbit NFkB p65 Polyclonal Antibody | anti-NFkB p65 antibody

NFkB p65 Antibody

Cat. Num. AAA840164

• Gene Names: Rela; p65
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: NFkB p65; Polyclonal Antibody; NFkB p65 Antibody; RELA; NFKB3; MGC131774; anti-NFkB p65 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.2 mg/ml) affinity purified rabbit anti-NFkB polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 25% glycerol, 0.5% BSA, 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.2 mg/ml (varies by lot)
• Sequence Length: 549

• Applicable Applications for anti-NFkB p65 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting (0.5-4 ug/ml), immunocytochemistry (10-20ug/ml). However, the optimal conditions should be determined individually. The antibody detects primarily NFkB p65 in samples of human, mouse, and rat origins.
• Immunogen: Synthetic peptide surrounding amino acid 274 of mouse NFkB p65
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-NFkB p65 antibody

Background: NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 6677709
• NCBI GeneID: 19697
• NCBI Accession #: NP_033071
• NCBI GenBank Nucleotide #: NM_009045.4
• Molecular Weight: 59,061 Da
• NCBI Official Full Name: transcription factor p65
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; enzyme binding; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cellular response to stress; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; defense response; negative regulation of transcription from RNA polymerase II promoter; activation of NF-kappaB transcription factor; response to organic substance; regulation of transcription, DNA-dependent; hair follicle development; positive regulation of cell proliferation; inflammatory response; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; positive regulation of interleukin-12 biosynthetic process; positive regulation of chondrocyte differentiation; response to bacterium; response to muramyl dipeptide; response to cytokine stimulus; regulation of inflammatory response; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process; negative regulation of apoptosis