Rabbit anti-Mouse NFkB Polyclonal Antibody | anti-NFkB antibody

RABBIT ANTI NFkB (p65)

Cat. Num. AAA224892

• Gene Names: Rela; p65
• Reactivity: Mouse
• Applications: Immunocytochemistry, Western Blot
• Synonyms: NFkB; Polyclonal Antibody; RABBIT ANTI NFkB (p65); anti-NFkB antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NFkB (p65)
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 0.2 mg/ml (varies by lot)
• Sequence Length: 549

• Applicable Applications for anti-NFkB antibody: Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: Immunocytochemistry: 10; 20 ug/ml; Western Blot: 0.5; 4.0 ug/ml
• Reactivity Notes: Human, Rat
• Preservative Stabilisers: 25% Glycerol, 0.5% Bovine Serum Albumin, 0.01% Thiomersal
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at -20 degree C Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of Jurkat cell lysate probed with Rabbit anti NFkB p65 antibody)

Related Product Information for anti-NFkB antibody

Rabbit anti NFkB p65 antibody recognizes Nuclear factor NF-kappa-B p65 subunit also known as Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 or RelA. NF-kappa-B p105 and p100 subunits are large precursor proteins that are cleaved by the ubiquitin/proteasome pathway into the mature NF-kappa-B p50 and p52 subunits (NF-kappa-B p105 cleavage results in the p50 unit, while cleavage of NF-kappa-B p100 results in the p52 subunit). Rel protein family members interact with p52 and p50 to form transcription factors. In the absence of stimulation an I-kappa-B protein mediated sequestration mechanism ensures that the transcription factors remain in the cytoplasm. Upon stimulation, I-kappa-B proteins become phosphorylated, which results in their ubiquitylation and subsequent degradation by the 26S proteasome.The NF-kappa-B pathway plays a critical in mediating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 417926
• NCBI GeneID: 19697
• UniProt Accession #: Q04207; Q62025
• Molecular Weight: 59,061 Da
• NCBI Official Full Name: Transcription factor p65
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: Rela
• NCBI Official Synonym Symbols: p65
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: Rela
• UniProt Synonym Gene Names: Nfkb3
• UniProt Entry Name: TF65_MOUSE

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Transcription factor; DNA-binding; Nuclear receptor co-regulator

Cellular Component: cytoplasm; cytosol; nucleus; protein complex; transcription factor complex

Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; enzyme binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; defense response; hair follicle development; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; liver development; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 biosynthetic process; positive regulation of Schwann cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; regulation of transcription, DNA-dependent; response to bacterium; response to cytokine stimulus; response to muramyl dipeptide; response to organic substance; response to UV-B; transcription from RNA polymerase II promoter; transcription, DNA-dependent