Canine Nuclear factor NF-kappa-B p105 subunit ELISA Kit | NFKB1/p105 elisa kit

Canine Nuclear factor NF-kappa-B p105 subunit ELISA Kit

Cat. Num. AAA751072

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Reactivity: Canine
• Synonyms: Nuclear factor NF-kappa-B p105 subunit; Canine Nuclear factor NF-kappa-B p105 subunit ELISA Kit; NFKB1/p105 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFKB1/p105 elisa kit

Intended Uses: This NFKB1/p105 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NFKB1/p105. This ELISA kit for research use only!

Principle of the Assay: NFKB1/p105 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NFKB1/p105 antibody and an NFKB1/p105-HRP conjugate. The assay sample and buffer are incubated together with NFKB1/p105-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NFKB1/p105 concentration since NFKB1/p105 from samples and NFKB1/p105-HRP conjugate compete for the anti-NFKB1/p105 antibody binding site. Since the number of sites is limited, as more sites are occupied by NFKB1/p105 from the sample, fewer sites are left to bind NFKB1/p105-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFKB1/p105 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34577122
• NCBI GeneID: 4790
• NCBI Accession #: NP_003989.2
• NCBI GenBank Nucleotide #: NM_003998.3
• Molecular Weight: 85,520 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 1
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit; NF-kappabeta; DNA binding factor KBF1; DNA-binding factor KBF1; nuclear factor NF-kappa-B p50 subunit; nuclear factor kappa-B DNA binding subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN

NCBI Description

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; double-stranded DNA binding; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of cholesterol transport; negative regulation of cellular protein metabolic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; membrane protein intracellular domain proteolysis; MyD88-independent toll-like receptor signaling pathway; negative regulation of interleukin-12 biosynthetic process; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to copper ion; negative regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; toll-like receptor 9 signaling pathway; negative regulation of apoptosis