Testing Data
(Images: Adult rat brain cortex (Left) and striatum (Right) sections (45 muM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with our Calbindin (1:1,000, red), and our monoclonal mouse Fox3/NeuN. Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positvie interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express Fox3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.)
Calbindin Antibody | anti-CALB1 antibody
Calbindin, affinity purified, chicken
Testing Data
(Images: Adult rat brain cortex (Left) and striatum (Right) sections (45 muM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with our Calbindin (1:1,000, red), and our monoclonal mouse Fox3/NeuN. Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positvie interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express Fox3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.)
Testing Data
(Images: Adult rat brain cortex (Left) and striatum (Right) sections (45 muM; fixed by transcardial perfusion with 4% paraformaldehyde) were stained with our Calbindin (1:1,000, red), and our monoclonal mouse Fox3/NeuN. Calbindin labels a subset of sparsely-distributed interneurons (calbindin-positvie interneurons) in the cortex (Left), and more densely-distributed neurons in the striatum (Right). Since neurons also express Fox3/NeuN, calbindin-positive cells appear to be gold to yellow. Insets are high magnification images of boxed area of each image. Blue is Dapi nucleus staining.)
Testing Data
(Image: Western blot analysis of Calbindin. Blots of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with Calbindin at 1:5,000. Lane1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin. In rat brain lysates, this antibody recognizes a clear band at 30 kDa which represents calbindin and it reacts only with calbindin protein, and not other calcium-binding proteins. The band at ~60 kDa is most likely the dimer of calbindin.)
Testing Data
(Image: Western blot analysis of Calbindin. Blots of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with Calbindin at 1:5,000. Lane1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin. In rat brain lysates, this antibody recognizes a clear band at 30 kDa which represents calbindin and it reacts only with calbindin protein, and not other calcium-binding proteins. The band at ~60 kDa is most likely the dimer of calbindin.)
The function of calbindin-1 appears to be primarily buffering the Ca2+ level in cells. The affinity of calbindin for Ca2+ is low at the typical resting cytoplasmic Ca2+ level of around 100 nM, and the protein only binds Ca2+ significantly when levels increase greatly. Accordingly, it is widely thought that the primary function of this protein is to act as a Ca2+ buffer. Buffering Ca2+ is important, as uncontrolled increases in the level of this cation can lead to both apoptosis due to Ca2+stimulated release of proteins from mitochondria and necrosis due to the activation of Ca2+dependent proteases.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]
Uniprot Description
CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 8q21.3
Cellular Component: axon; cell soma; cytosol; dendrite; nucleus; synapse
Molecular Function: calcium ion binding; protein binding; zinc ion binding
Biological Process: cellular protein metabolic process; cytosolic calcium ion homeostasis; long-term memory; short-term memory
Research Articles on CALB1
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Product Notes
The CALB1 calb1 (Catalog #AAA555649) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Calbindin, affinity purified, chicken reacts with Bovine, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calbindin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the CALB1 calb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calbindin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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