Rabbit anti-Human RASA1 Polyclonal Antibody | anti-RASA1 antibody

RASA1, CT (RASA1, RASA, Ras GTPase-activating protein 1, Ras p21 protein activator, p120GAP)

Cat. Num. AAA647478

• Gene Names: RASA1; GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RASA1; Polyclonal Antibody; CT (RASA1; RASA; Ras GTPase-activating protein 1; Ras p21 protein activator; p120GAP); Anti -RASA1; anti-RASA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RASA1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: RASA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 965-993 amino acids from the C-terminal region of human RASA1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) using MBS647478.This demonstrates the RASA1 antibody detected the RASA1 protein (arrow).)

Related Product Information for anti-RASA1 antibody

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.

Product Categories/Family for anti-RASA1 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 12545406
• NCBI GeneID: 5921
• NCBI Accession #: NP_072179.1
• NCBI GenBank Nucleotide #: NM_022650.2
• UniProt Accession #: P20936; Q9UDI1; B2R6W3
• Molecular Weight: 116,403 Da
• NCBI Official Full Name: ras GTPase-activating protein 1 isoform 2
• NCBI Official Synonym Full Names: RAS p21 protein activator (GTPase activating protein) 1
• NCBI Official Symbol: RASA1
• NCBI Official Synonym Symbols: GAP; PKWS; RASA; CMAVM; CM-AVM; RASGAP; p120GAP; p120RASGAP
• NCBI Protein Information: ras GTPase-activating protein 1; triphosphatase-activating protein
• UniProt Protein Name: Ras GTPase-activating protein 1
• Protein Family: Ras GTPase-activating protein
• UniProt Gene Name: RASA1
• UniProt Synonym Gene Names: RASA; GAP; GTPase-activating protein; RasGAP
• UniProt Entry Name: RASA1_HUMAN

NCBI Description

The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]

Uniprot Description

Function: Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1. Ref.4 Ref.6

Subunit structure: Interacts with SQSTM1. Interacts with SPSB1; the interaction does not promote degradation. Interacts with CAV2 (tyrosine phosphorylated form). Directly interacts with NCK1. Interacts with PDGFRB (tyrosine phosphorylated). Interacts (via SH2 domain) with the 'Tyr-9' phosphorylated form of PDPK1. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.15

Subcellular location: Cytoplasm Ref.4.

Tissue specificity: In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level). Ref.4

Post-translational modification: The N-terminus is blocked.Phosphorylated by SRC and LCK. The phosphorylation SRC inhibits its ability to stimulate the Ras-GTPase activity, whereas phosphorylation by LCK does not display any effect on stimulation activity. Ref.6

Involvement in disease: Mutations in the SH2 domain of RASA seem to be oncogenic and cause basal cell carcinomas.Capillary malformation-arteriovenous malformation (CMAVM) [MIM:608354]: Disorder characterized by atypical capillary malformations that are multiple, small, round to oval in shape and pinkish red in color. These capillary malformations are associated with either arteriovenous malformation, arteriovenous fistula, or Parkes Weber syndrome.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.21Parkes Weber syndrome (PKWS) [MIM:608355]: Disorder characterized by a cutaneous flush with underlying multiple micro-arteriovenous fistulas, in association with soft tissue and skeletal hypertrophy of the affected limb.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 C2 domain.Contains 1 PH domain.Contains 1 Ras-GAP domain.Contains 2 SH2 domains.Contains 1 SH3 domain.