Transcription factor p65 Recombinant Protein | NFKB3 recombinant protein

Recombinant Human Transcription factor p65 protein

• Gene Names: RELA; p65; NFKB3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transcription factor p65; Recombinant Human Transcription factor p65 protein; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; NFKB3 recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 1-210aa; Partial
• Sequence: MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGD
• Sequence Length: 548

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for NFKB3 recombinant protein

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with mbers of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells

Product Categories/Family for NFKB3 recombinant protein

Immunology

References

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Med. 200:107-113(2004) Identification of beta-arrestin2 as a G protein-coupled receptor-stimulated regulator of NF-kappaB pathways.Gao H., Sun Y., Wu Y., Luan B., Wang Y., Qu B., Pei G.Mol. Cell 14:303-317(2004) The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis.Garkavtsev I., Kozin S.V., Chernova O., Xu L., Winkler F., Brown E., Barnett G.H., Jain R.K.Nature 428:328-332(2004) Regulation of NF-kappaB and p53 through activation of ATR and Chk1 by the ARF tumour suppressor.Rocha S., Garrett M.D., Campbell K.J., Schumm K., Perkins N.D.EMBO J. 24:1157-1169(2005) IKKbeta phosphorylates p65 at S468 in transactivation domain 2.Schwabe R.F., Sakurai H.FASEB J. 19:1758-1760(2005) cis-acting, element-specific transcriptional activity of differentially phosphorylated nuclear factor-kappa B.Anrather J., Racchumi G., Iadecola C.J. Biol. 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U.S.A. 102:5138-5143(2005) Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B.Reimers K., Buchholz K., Werchau H.Virology 331:260-268(2005) Activation of the nuclear factor kappaB pathway by astrocyte elevated gene-1 implications for tumor progression and metastasis.Emdad L., Sarkar D., Su Z.-Z., Randolph A., Boukerche H., Valerie K., Fisher P.B.Cancer Res. 66:1509-1516(2006) Human ubiquitin specific protease 31 is a deubiquitinating enzyme implicated in activation of nuclear factor-kappaB.Tzimas C., Michailidou G., Arsenakis M., Kieff E., Mosialos G., Hatzivassiliou E.G.Cell. Signal. 18:83-92(2006) Inducible phosphorylation of NF-kappa B p65 at serine 468 by T cell costimulation is mediated by IKK epsilon.Mattioli I., Geng H., Sebald A., Hodel M., Bucher C., Kracht M., Schmitz M.L.J. Biol. Chem. 281:6175-6183(2006) Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing histone deacetylase 1-mediated deacetylation of RelA/p65 and promoting apoptosis.Liu Y., Smith P.W., Jones D.R.Mol. Cell. Biol. 26:8683-8696(2006) Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17.Miao F., Li S., Chavez V., Lanting L., Natarajan R.Mol. Endocrinol. 20:1562-1573(2006) IL-1 receptor-associated kinase 1 is critical for latent membrane protein 1-induced p65/RelA serine 536 phosphorylation and NF-kappaB activation.Song Y.J., Jen K.Y., Soni V., Kieff E., Cahir-McFarland E.Proc. Natl. Acad. Sci. U.S.A. 103:2689-2694(2006) Nine-amino-acid transactivation domain establishment and prediction utilities.Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.Genomics 89:756-768(2007) UXT is a novel and essential cofactor in the NF-kappaB transcriptional enhanceosome.Sun S., Tang Y., Lou X., Zhu L., Yang K., Zhang B., Shi H., Wang C.J. Cell Biol. 178:231-244(2007) The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF-kappaB through its N-terminal fragment.Chae J.J., Wood G., Richard K., Jaffe H., Colburn N.T., Masters S.L., Gumucio D.L., Shoham N.G., Kastner D.L.Blood 112:1794-1803(2008) Functional characterization of the atopy-associated gene PHF11.Clarke E., Rahman N., Page N., Rolph M.S., Stewart G.J., Jones G.J.J. Allergy Clin. Immunol. 121:1148-1154(2008) AKIP1 enhances NF-kappaB-dependent gene expression by promoting the nuclear retention and phosphorylation of p65.Gao N., Asamitsu K., Hibi Y., Ueno T., Okamoto T.J. Biol. Chem. 283:7834-7843(2008) The DEAD-box RNA helicase DDX1 interacts with RelA and enhances nuclear factor kappaB-mediated transcription.Ishaq M., Ma L., Wu X., Mu Y., Pan J., Hu J., Hu T., Fu Q., Guo D.J. Cell. Biochem. 106:296-305(2009) Brd4 coactivates transcriptional activation of NF-kappaB via specific binding to acetylated RelA.Huang B., Yang X.D., Zhou M.M., Ozato K., Chen L.F.Mol. Cell. Biol. 29:1375-1387(2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.Science 325:834-840(2009) A novel LZAP-binding protein, NLBP, inhibits cell invasion.Kwon J., Cho H.J., Han S.H., No J.G., Kwon J.Y., Kim H.J. Biol. Chem. 285:12232-12240(2010) SIRT2 regulates NF-kappaB dependent gene expression through deacetylation of p65 Lys310.Rothgiesser K.M., Erener S., Waibel S., Luscher B., Hottiger M.O.J. Cell Sci. 123:4251-4258(2010) Zinc finger protein Gfi1 controls the endotoxin-mediated Toll-like receptor inflammatory response by antagonizing NF-kappaB p65.Sharif-Askari E., Vassen L., Kosan C., Khandanpour C., Gaudreau M.C., Heyd F., Okayama T., Jin J., Rojas M.E., Grimes H.L., Zeng H., Moroy T.Mol. Cell. Biol. 30:3929-3942(2010) Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011) NF-kappaB repression by PIAS3 mediated RelA SUMOylation.Liu Y., Bridges R., Wortham A., Kulesz-Martin M.PLoS ONE 7:E37636-E37636(2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) C11orf95-RELA fusions drive oncogenic NF-kappaB signalling in ependymoma.Parker M., Mohankumar K.M., Punchihewa C., Weinlich R., Dalton J.D., Li Y., Lee R., Tatevossian R.G., Phoenix T.N., Thiruvenkatam R., White E., Tang B., Orisme W., Gupta K., Rusch M., Chen X., Li Y., Nagahawhatte P. , Hedlund E., Finkelstein D., Wu G., Shurtleff S., Easton J., Boggs K., Yergeau D., Vadodaria B., Mulder H.L., Becksfort J., Becksford J., Gupta P., Huether R., Ma J., Song G., Gajjar A., Merchant T., Boop F., Smith A.A., Ding L., Lu C., Ochoa K., Zhao D., Fulton R.S., Fulton L.L., Mardis E.R., Wilson R.K., Downing J.R., Green D.R., Zhang J., Ellison D.W., Gilbertson R.J.Nature 506:451-455(2014) Structure of an IkappaBalpha/NF-kappaB complex.Jacobs M.D., Harrison S.C.Cell 95:749-758(1998) +Additional computationally mapped references.<p>Provides general information on the entry.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: 51kD
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel avian reticuloendotheliosis viral oncogene homolog A
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• Protein Family: Transcription factor
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; transcription factor complex

Molecular Function: actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein complex binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding

Biological Process: acetaldehyde metabolic process; activation of NF-kappaB transcription factor; aging; cellular defense response; cytokine and chemokine mediated signaling pathway; defense response to virus; hair follicle development; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; liver development; membrane protein intracellular domain proteolysis; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; organ morphogenesis; positive regulation of cell proliferation; positive regulation of chondrocyte differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-12 biosynthetic process; positive regulation of Schwann cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to amino acid stimulus; response to cAMP; response to cobalamin; response to drug; response to insulin stimulus; response to morphine; response to muramyl dipeptide; response to organic substance; response to progesterone stimulus; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter; viral reproduction