Rabbit NFkappaB-p100/p52 Polyclonal Antibody | anti-NFkappaB-p100/p52 antibody

NFkappaB-p100/p52 (Ab-866) Antibody

Cat. Num. AAA9413642

• Gene Names: NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Synonyms: NFkappaB-p100/p52; Polyclonal Antibody; NFkappaB-p100/p52 (Ab-866) Antibody; NFkB-p100/p52 (Ab-866) Antibody; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; NFKB2; anti-NFkappaB-p100/p52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total NFkB-p100 protein.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 900

• Applicable Applications for anti-NFkappaB-p100/p52 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around aa.864~868 (E-D-S-A-Y) derived from Human NFkB-p100.
• Target Name: NFkB-p100
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from HT29 cells using NFkB-p100(Ab-866) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p100(Ab-866) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p100(Ab-866) Antibody.)

Related Product Information for anti-NFkappaB-p100/p52 antibody

NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth.

Product Categories/Family for anti-NFkappaB-p100/p52 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 117320531
• NCBI GeneID: 4791
• NCBI Accession #: NP_001070962.1
• NCBI GenBank Nucleotide #: NM_001077494.3
• UniProt Accession #: Q00653; Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83; AAB23437
• Molecular Weight: 96,678 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• NCBI Official Symbol: NFKB2
• NCBI Official Synonym Symbols: p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• UniProt Gene Name: NFKB2
• UniProt Synonym Gene Names: LYT10; Lyt10
• UniProt Entry Name: NFKB2_HUMAN

NCBI Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; follicular dendritic cell differentiation; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; positive regulation of interferon type I production; germinal center formation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway

Disease: Immunodeficiency, Common Variable, 10