Western Blot (WB)
(RFT1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RFT1 antibody detected the RFT1 protein (arrow).)
Rabbit anti-Human RFT1 Polyclonal Antibody | anti-RFT1 antibody
RFT1 Antibody (C-term)
Western Blot (WB)
(RFT1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RFT1 antibody detected the RFT1 protein (arrow).)
Western Blot (WB)
(RFT1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RFT1 antibody detected the RFT1 protein (arrow).)
Immunohistochemistry (IHC)
(RFT1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RFT1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RFT1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RFT1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an enzyme which catalyzes the translocation of the Man(5)GlcNAc (2)-PP-Dol intermediate from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane in the pathway for the N-glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type In.[provided by RefSeq, Dec 2008]
Uniprot Description
RFT1: May be involved in N-linked oligosaccharide assembly. May participate in the translocation of oligosaccharide from the cytoplasmic side to the lumenal side of the endoplasmic reticulum membrane. Defects in RFT1 are the cause of congenital disorder of glycosylation type 1N (CDG1N). CDGs are a genetically heterogeneous group of autosomal recessive disorders caused by enzymatic defects in the synthesis and processing of asparagine (N)-linked glycans or oligosaccharides on glycoproteins. CDGs present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. Type 1 CDGs comprise defects in the assembly of the dolichol lipid-linked oligosaccharide chain and its transfer to the nascent protein. These disorders can be identified by a characteristic abnormal isoelectric focusing profile of plasma transferrin. Belongs to the RFT1 family.
Protein type: Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; Transporter; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: lipid transporter activity
Biological Process: cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate transport; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; glycolipid translocation
Disease: Congenital Disorder Of Glycosylation, Type In
Research Articles on RFT1
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Product Notes
The RFT1 rft1 (Catalog #AAA9205772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 513-541. The RFT1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RFT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the RFT1 rft1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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