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Western Blot (WB) (Western blot analysis of extracts of BxPC-3 cells, using VNN1 antibody.)

Rabbit anti-Human, Mouse VNN1 Polyclonal Antibody | anti-VNN1 antibody

VNN1 Antibody

Gene Names
VNN1; HDLCQ8; Tiff66
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
VNN1; Polyclonal Antibody; VNN1 Antibody; HDLCQ8; High density lipoprotein cholesterol level quantitative trait locus 8; included; Pantetheinase; Pantetheine hydrolase; Tiff66; V-1; Vanin 1; Vanin-1; Vannin 1; Vascular non inflammatory molecule 1; Vascular non-inflammatory molecule 1; VNN 1; VNN1_HUMAN; anti-VNN1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
VNN1 antibody detects endogenous levels of VNN1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
513
Applicable Applications for anti-VNN1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human VNN1.
Subcellular Location
Cell Membrane
Tissue Specificity
Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (83%), Sheep (100%), Rabbit (100%), Dog (92%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of BxPC-3 cells, using VNN1 antibody.)

Western Blot (WB) (Western blot analysis of extracts of BxPC-3 cells, using VNN1 antibody.)

Immunohistochemistry (IHC)

(MBS9603469 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Immunohistochemistry (IHC) (MBS9603469 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-VNN1 antibody
Function: Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
Subunit Structure: Monomer.
Similarity: Belongs to the carbon-nitrogen hydrolase superfamily. BTD/VNN family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 55-72 kDa
Predicted: 58 kDa
NCBI Official Full Name
pantetheinase
NCBI Official Synonym Full Names
vanin 1
NCBI Official Symbol
VNN1
NCBI Official Synonym Symbols
HDLCQ8; Tiff66
NCBI Protein Information
pantetheinase
UniProt Protein Name
Pantetheinase
Protein Family
UniProt Gene Name
VNN1
UniProt Synonym Gene Names
Vanin-1

NCBI Description

This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. No biotinidase activity has been demonstrated for any of the vanin proteins, however, they possess pantetheinase activity, which may play a role in oxidative-stress response. This protein, like its mouse homolog, is likely a GPI-anchored cell surface molecule. The mouse protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. This gene lies in close proximity to, and in the same transcriptional orientation as, two other vanin genes on chromosome 6q23-q24. [provided by RefSeq, Feb 2009]

Uniprot Description

Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.

Research Articles on VNN1

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Product Notes

The VNN1 vnn1 (Catalog #AAA9603469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VNN1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VNN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the VNN1 vnn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VNN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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