Rabbit NFkappaB-p100/p52 Polyclonal Antibody | anti-NFkappaB-p100/p52 antibody

NFkappaB-p100/p52(Phospho-Ser866) Antibody

Cat. Num. AAA9413200

• Gene Names: NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: NFkappaB-p100/p52; Polyclonal Antibody; NFkappaB-p100/p52(Phospho-Ser866) Antibody; NFkB-p100/p52(Phospho-Ser866) Antibody; p52; p105; H2TF1; LYT10; NF-kB2; anti-NFkappaB-p100/p52 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of NFŽ degree B-p100 only when phosphorylated at serine 866.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 900

• Applicable Applications for anti-NFkappaB-p100/p52 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of serine 866(E-D-S(p)-A-Y) derived from Human NFkappaB-p100.
• Modification: Phospho-Ser866
• Target Name: NFkappaB-p100
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from ovary cancer cells using NF-kappaB p100 (phospho-Ser866) antibody ().)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p100 (phospho-ser866) antibody (, Red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NF-kappaB p100 (phospho- ser866) antibody ().)

Related Product Information for anti-NFkappaB-p100/p52 antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Product Categories/Family for anti-NFkappaB-p100/p52 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 117320531
• NCBI GeneID: 4791
• NCBI Accession #: NP_001070962.1
• NCBI GenBank Nucleotide #: NM_001077494.3
• UniProt Accession #: Q00653; Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83; AAB23437
• Molecular Weight: 96,678 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• NCBI Official Symbol: NFKB2
• NCBI Official Synonym Symbols: p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• UniProt Gene Name: NFKB2
• UniProt Synonym Gene Names: LYT10; Lyt10
• UniProt Entry Name: NFKB2_HUMAN

NCBI Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protein type: DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; follicular dendritic cell differentiation; MyD88-independent toll-like receptor signaling pathway; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; positive regulation of interferon type I production; germinal center formation; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway

Disease: Immunodeficiency, Common Variable, 10