p52 protein

p52 protein

Cat. Num. AAA8526792

• Gene Names: NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
• Purity: > 95% as determined by reducing SDS-PAGE
• Synonyms: p52; p52 protein

• Purity/Purification: > 95% as determined by reducing SDS-PAGE
• Form/Format: Supplied as a 0.2 μ filtered solution of 20mM Tris-HCl, 10% Glycerol, pH 8.0
• Sequence Length: 900

• Preparation and Storage: Store for ≤ -70°C, stable for 6 months afer receipt. Store for ≤ -70°C, stable for 3 months under sterile conditions after opening. Avoid repeated freeze/thaw cycles

SDS-PAGE

Related Product Information for p52 protein

Recombinant human 52kDa FK506 Binding Protein is produced by ouy E.coli expression system and the target encoding Met1-Ala459 is expressed with a 6His tag at the C terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 117320531
• NCBI GeneID: 4791
• NCBI Accession #: Q02790
• NCBI GenBank Nucleotide #: NM_001077494.3
• UniProt Accession #: Q00653; Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83
• Molecular Weight: Molecular Mass: 52.8 kDa; AP Molecular Mass: 50-60 kDa, reducing conditions.
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor kappa B subunit 2
• NCBI Official Symbol: NFKB2
• NCBI Official Synonym Symbols: p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• Protein Family: Sporozoite surface protein
• UniProt Gene Name: NFKB2
• UniProt Synonym Gene Names: LYT10; Lyt10

NCBI Description

This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.