Testing Data
(A.. Mouse bEND.3 were stained with M1276-91C (filled histogram) or isotype control (open histogram).)
Goat anti-Rat Neuropilin-1 Polyclonal Antibody | anti-NRP1 antibody
Neuropilin-1 (NRP1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (Carboxyfluorescein)
Purified by affinity chromatography.
Purified by affinity chromatography.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated on bEND.3 cells and PC-12 cells.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells
twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
4. The cells were resuspended in Flow Cytometry Staining
Buffer for analysis by Flow Cytometry. As a control for this
analysis, cells in a separate tube should be treated with
CFS-labeled goat IgG antibody.
Testing Data
(A.. Mouse bEND.3 were stained with M1276-91C (filled histogram) or isotype control (open histogram).)
Testing Data
(A.. Mouse bEND.3 were stained with M1276-91C (filled histogram) or isotype control (open histogram).)
Testing Data
(B.. Rat PC-12 cells were stained with M1276-91C (filled histogram) or isotype control (open histogram).)
Testing Data
(B.. Rat PC-12 cells were stained with M1276-91C (filled histogram) or isotype control (open histogram).)
NCBI and Uniprot Product Information
NCBI Description
NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.[supplied by OMIM]
Uniprot Description
NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10p12
Cellular Component: extracellular space; cell surface; focal adhesion; early endosome; integral to membrane; cytosol; growth cone; cell soma; axon; neurofilament; plasma membrane; cytoplasmic vesicle; receptor complex
Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; growth factor binding; cytokine binding; metal ion binding; coreceptor activity
Biological Process: facial nerve structural organization; axon guidance; neuron migration; vestibulocochlear nerve structural organization; platelet-derived growth factor receptor signaling pathway; negative regulation of axon extension involved in axon guidance; positive regulation of smooth muscle cell migration; signal transduction; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; positive chemotaxis; cell-cell signaling; branchiomotor neuron axon guidance; dendrite development; response to wounding; angiogenesis; negative regulation of neuron apoptosis; trigeminal nerve structural organization; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; axon extension involved in axon guidance; patterning of blood vessels; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; cell migration during sprouting angiogenesis; otic placode formation; positive regulation of axon extension involved in axon guidance; artery morphogenesis; nerve development; positive regulation of endothelial cell proliferation; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance
Research Articles on NRP1
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Product Notes
The NRP1 nrp1 (Catalog #AAA620404) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Neuropilin-1 (NRP1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (Carboxyfluorescein) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuropilin-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated on bEND.3 cells and PC-12 cells. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT. 3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer. 4. The cells were resuspended in Flow Cytometry Staining Buffer for analysis by Flow Cytometry. As a control for this analysis, cells in a separate tube should be treated with CFS-labeled goat IgG antibody. Researchers should empirically determine the suitability of the NRP1 nrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuropilin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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