Mouse AQP9 (Aquaporin-9) ELISA Kit | Aqp9 elisa kit

Mouse AQP9 (Aquaporin-9) ELISA Kit

Cat. Num. AAA7612391

• Gene Names: Aqp9; AQP-9; AI266899; 1700020I22Rik
• Reactivity: Mouse
• Synonyms: AQP9 (Aquaporin-9); Mouse AQP9 (Aquaporin-9) ELISA Kit; Aquaporin-9; AQP-9; Aquaglyceroporin-9; Aqp9; Aqp9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aqp9. No significant cross-reactivity or interference between Aqp9 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aqp9 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 47116925
• NCBI GeneID: 64008
• UniProt Accession #: Q9JJJ3
• Molecular Weight: 31,764 Da
• NCBI Official Full Name: Aquaporin-9
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: Aqp9
• NCBI Official Synonym Symbols: AQP-9; AI266899; 1700020I22Rik
• NCBI Protein Information: aquaporin-9; aquaglyceroporin-9
• UniProt Protein Name: Aquaporin-9
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp9
• UniProt Synonym Gene Names: AQP-9
• UniProt Entry Name: AQP9_MOUSE

NCBI Description

This gene encodes a member of the aquaglyceroporin subfamily of aquaporins. This protein transports water, glycerol, urea, purines and pyrimidines and plays a role in glycerol metabolism and osteoclast differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, integral; Channel, misc.; Transporter, aquaporin family; Membrane protein, multi-pass; Transporter

Cellular Component: membrane; intracellular membrane-bound organelle; cell; integral to plasma membrane; basolateral plasma membrane; integral to membrane

Molecular Function: purine transmembrane transporter activity; pyrimidine transmembrane transporter activity; transporter activity; urea transmembrane transporter activity; water channel activity; glycerol channel activity

Biological Process: polyol transport; glycerol transport; cellular water homeostasis; nucleobase, nucleoside, nucleotide and nucleic acid transport; amine transport; cellular response to stress; water transport; purine transport; response to organic substance; response to mercury ion; transport; pyrimidine transport; canalicular bile acid transport; transmembrane transport