Neuropilin-1 Active Protein | NRP1 active protein

Neuropilin-1 Protein, Human, Recombinant (ECD, V179A, hFc Tag)

Cat. Num. AAA8119941

• Gene Names: NRP1; NP1; NRP; BDCA4; CD304; VEGF165R
• Purity: >85% as determined by SDS-PAGE
• Synonyms: Neuropilin-1; Neuropilin-1 Protein; Human; Recombinant (ECD; V179A; hFc Tag); Human Neuropilin-1/NRP1 Protein (ECD; Fc Tag); BDCA4 Protein; CD304 Protein; NP1 Protein; NRP Protein; VEGF165R Protein; neuropilin 1; NRP1 active protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys644

• Species: Human
• Activity: Measured by its ability to bind with recombinant human VEGF165 in a functional ELISA.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 22
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the human NRP1 isoform b (NP_001019799.1, with natural variant Val 179 Ala)(Met1-Lys644) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for NRP1 active protein

Background: Neuropilin is a type I transmembrane protein and the molecular mass is 120kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

References

Nakamura F, et al. (2002) Structural and functional relation of neuropilins. Adv Exp Med Biol. 515: 55-69.Romeo PH, et al. (2002) Neuropilin-1 in the immune system. Adv Exp Med Biol. 515: 49-54.Klagsbrun M, et al. (2002) The role of neuropilin in vascular and tumor biology. Adv Exp Med Biol. 515: 33-48.Staton CA, et al. (2007) Neuropilins in physiological and pathological angiogenesis. J Pathol. 212(3): 237-48.Bagri A, et al. (2009) Neuropilins in tumor biology. Clin Cancer Res. 15(6): 1860-4.

NCBI and Uniprot Product Information

• NCBI GI #: 66912178
• NCBI GeneID: 8829
• NCBI Accession #: NP_001019799.1
• NCBI GenBank Nucleotide #: NM_001024628.2
• UniProt Accession #: O14786; O60461; Q5T7F2; Q5T7F3; Q86T59; Q96IH5; B0LPG9
• Molecular Weight: 923
• NCBI Official Full Name: neuropilin-1 isoform b
• NCBI Official Synonym Full Names: neuropilin 1
• NCBI Official Symbol: NRP1
• NCBI Official Synonym Symbols: NP1; NRP; BDCA4; CD304; VEGF165R
• NCBI Protein Information: neuropilin-1; transmembrane receptor; vascular endothelial cell growth factor 165 receptor
• UniProt Protein Name: Neuropilin-1
• Protein Family: Neuropilin
• UniProt Gene Name: NRP1
• UniProt Synonym Gene Names: NRP; VEGF165R
• UniProt Entry Name: NRP1_HUMAN

Uniprot Description

NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: extracellular space; cell surface; focal adhesion; growth cone; cell soma; axon; early endosome; neurofilament; integral to membrane; plasma membrane; cytoplasmic vesicle; cytosol; receptor complex

Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; metal ion binding; growth factor binding; cytokine binding; coreceptor activity

Biological Process: axon guidance; facial nerve structural organization; neuron migration; vestibulocochlear nerve structural organization; platelet-derived growth factor receptor signaling pathway; positive regulation of smooth muscle cell migration; negative regulation of axon extension involved in axon guidance; signal transduction; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; cell-cell signaling; positive chemotaxis; branchiomotor neuron axon guidance; dendrite development; response to wounding; negative regulation of neuron apoptosis; angiogenesis; trigeminal nerve structural organization; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; patterning of blood vessels; axon extension involved in axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; cell migration during sprouting angiogenesis; otic placode formation; positive regulation of axon extension involved in axon guidance; artery morphogenesis; positive regulation of endothelial cell proliferation; nerve development; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance