Testing Data
(P-peptide-+)
Neutrophil Cytosol Factor 1 Antibody | anti-NCF-1 antibody
Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody
Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Neutrophil Cytosol Factor 1; Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; NCF1; Neutrophil NADPH oxidase factor 1; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; P47 phox; p47-phox; anti-NCF-1 antibody
Reactivity
Human, Mouse, Rat
Specificity
Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 328.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
390
Applicable Applications for anti-NCF-1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of serine 328 (R-N-SP-V-R).
All Sites
Human: Ser328
Mouse: Ser329
Rat: Ser329
Mouse: Ser329
Rat: Ser329
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody. Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human lymph node tissue using Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody. Neutrophil Cytosol Factor 1 (Phospho-Ser328) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Product Categories/Family for anti-NCF-1 antibody
References
Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989). Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989). Lomax K.J., Science 245:409-412(1989).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,811 Da
NCBI Official Full Name
neutrophil cytosol factor 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1
NCBI Official Symbol
NCF1
NCBI Official Synonym Symbols
NCF1A; NOXO2; p47phox; SH3PXD1A
NCBI Protein Information
neutrophil cytosol factor 1
UniProt Protein Name
Neutrophil cytosol factor 1
Protein Family
UniProt Gene Name
NCF1
UniProt Synonym Gene Names
NOXO2; SH3PXD1A; NCF-1
NCBI Description
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
Uniprot Description
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Research Articles on NCF-1
Similar Products
Product Notes
The NCF-1 ncf1 (Catalog #AAA859900) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neutrophil Cytosol Factor 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the NCF-1 ncf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neutrophil Cytosol Factor 1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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