Western Blot (WB)
((0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Neurofascin Polyclonal Antibody | anti-NFASC antibody
Goat anti-Neurofascin (aa877-890) Antibody
Western Blot: Approx 170kDa band observed in Human Lung and Rat Brain lysates (calculated MW of 138kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem.2009 Oct 16;284(42): 28533-42. PMID: 19666467). Recommended concentration: 0.5-2ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]
Uniprot Description
neurofascin: Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 13 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: focal adhesion; integral to membrane; intracellular; myelin sheath; paranode region of axon; plasma membrane
Molecular Function: protein binding; protein domain specific binding
Biological Process: axon guidance; clustering of voltage-gated sodium channels; heterotypic cell-cell adhesion; myelination; paranodal junction assembly; peripheral nervous system development; synapse organization and biogenesis; transmission of nerve impulse
Research Articles on NFASC
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Product Notes
The NFASC nfasc (Catalog #AAA422738) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Neurofascin (aa877-890) Antibody reacts with Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofascin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 170kDa band observed in Human Lung and Rat Brain lysates (calculated MW of 138kDa according to NP_001005388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kirschbaum et al, J Biol Chem.2009 Oct 16;284(42): 28533-42. PMID: 19666467). Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the NFASC nfasc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NFSPNQTKFT VQRT. It is sometimes possible for the material contained within the vial of "Neurofascin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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