Neurofascin Recombinant Protein | NFASC recombinant protein

Neurofascin Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121948

• Gene Names: NFASC; NF; NRCAML
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Neurofascin; Neurofascin Protein; Human; Recombinant (His Tag); Human NFASC/Neurofascin Protein (His Tag); NF Protein; NRCAML Protein; neurofascin; NFASC recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln939

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human NFASC (O94856-12)(Met1-Gln939) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NFASC recombinant protein

Background: NFASC (Neurofascin, also known as NRCAML, NEDCPMD, and NF) is a Protein Coding gene. 13 alternatively spliced human isoforms have been reported. NFASC belongs to the immunoglobulin superfamily, L1/neurofascin/NgCAM family. It contains 5 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. NFASC links the AIS extracellular matrix to the intracellular cytoskeleton. It is broadly expressed in the brain, kidney, and other tissues. Diseases associated with NFASC include Neurodevelopmental Disorder With Central And Peripheral Motor Dysfunction and Demyelinating Polyneuropathy.

References

Volkmer H. et al., 1992, J Cell Biol. 118 (1): 149-61. Burmeister M. et al., 1996, Mamm Genome. 7 (7): 558-9. Ango F. et al., 2004, Cell. 119 (2): 257-72.

NCBI and Uniprot Product Information

• NCBI GI #: 83304988
• NCBI GeneID: 23114
• UniProt Accession #: O94856; Q149P5; Q5T2F0; Q5T2F1; Q5T2F2; Q5T2F3; Q5T2F4; B2RNN8; B3KQZ1; B5MDP6; B5MDR6; B7ZMD8
• Molecular Weight: 1240
• NCBI Official Full Name: Neurofascin
• NCBI Official Synonym Full Names: neurofascin
• NCBI Official Symbol: NFASC
• NCBI Official Synonym Symbols: NF; NRCAML
• NCBI Protein Information: neurofascin; neurofascin homolog
• UniProt Protein Name: Neurofascin
• Protein Family: Neurofascin
• UniProt Gene Name: NFASC
• UniProt Synonym Gene Names: KIAA0756
• UniProt Entry Name: NFASC_HUMAN

NCBI Description

This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]

Uniprot Description

neurofascin: Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: focal adhesion; paranode region of axon; integral to membrane; plasma membrane; intracellular

Molecular Function: protein binding

Biological Process: myelination; heterotypic cell-cell adhesion; axon guidance; clustering of voltage-gated sodium channels; synapse organization and biogenesis; transmission of nerve impulse; peripheral nervous system development; paranodal junction assembly