Human Neurofascin (NFASC) ELISA Kit | NFASC elisa kit

Human Neurofascin (NFASC) ELISA Kit

Cat. Num. AAA455042

• Gene Names: NFASC; NF; NRCAML
• Reactivity: Human
• Synonyms: Neurofascin (NFASC); Human Neurofascin (NFASC) ELISA Kit; NFASC elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NFASC. No significant cross-reactivity or interference between NFASC and analogues was observed.
• Sequence Length: 1240

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.083ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NFASC were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NFASC were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for NFASC elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NFASC in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NFASC. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NFASC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NFASC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NFASC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 237858675
• NCBI GeneID: 23114
• NCBI Accession #: NP_001005388.2
• NCBI GenBank Nucleotide #: NM_001005388.2
• UniProt Accession #: O94856; Q149P5; Q5T2F0; Q5T2F1; Q5T2F2; Q5T2F3; Q5T2F4; B2RNN8; B3KQZ1; B5MDP6; B5MDR6; B7ZMD8
• Molecular Weight: 142,474 Da
• NCBI Official Full Name: neurofascin isoform 1
• NCBI Official Synonym Full Names: neurofascin
• NCBI Official Symbol: NFASC
• NCBI Official Synonym Symbols: NF; NRCAML
• NCBI Protein Information: neurofascin
• UniProt Protein Name: Neurofascin
• Protein Family: Neurofascin
• UniProt Gene Name: NFASC
• UniProt Synonym Gene Names: KIAA0756

NCBI Description

This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.[provided by RefSeq, May 2009]

Uniprot Description

Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions.