Western Blot (WB)
(Anti-NES rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:177 kDa Observed band size:250 kDa)
Rabbit anti-Human Nestin Polyclonal Antibody | anti-NES antibody
Anti-Nestin Antibody, Rabbit Polyclonal
IHC-P: 1:200-1:1000
IP: 1-5uL/mg of lysate
Western Blot (WB)
(Anti-NES rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:177 kDa Observed band size:250 kDa)
Western Blot (WB)
(Anti-NES rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: U251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:177 kDa Observed band size:250 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human NES in human glioma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human NES in human glioma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human NES in human neurilemmoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human NES in human neurilemmoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :250 kDa)
Immunoprecipitation (IP)
(NES was immunoprecipitated using: Lane A:0.5 mg U251MG Whole Cell Lysate 4 uL anti-NES rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NES rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 177 kDa Observed band size :250 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Uniprot Description
nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament
Molecular Function: intermediate filament binding; structural molecule activity
Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; cell projection morphogenesis; brain development; negative regulation of neuron apoptosis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
Research Articles on NES
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Product Notes
The NES nes (Catalog #AAA8103577) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Nestin Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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