Rabbit anti-Human PEG10 Polyclonal Antibody | anti-PEG10 antibody

Anti-PEG10 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108897

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PEG10; Polyclonal Antibody; Anti-PEG10 Antibody; Rabbit Polyclonal; PEG10 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-PEG10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PEG10
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PEG10 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:100-1:500; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human PEG10 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PEG10 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PEG10 rabbit polyclonal antibody at 1:500 dilution Lane A: HEK-293 Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PEG10 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human PEG10 in human adrenal gland with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of PEG10 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PEG10 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

Immunoprecipitation (IP)

(PEG10 was immunoprecipitated using: Lane A:0.5 mg HEK 293 Whole Cell Lysate 4 uL anti-PEG10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PEG10 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa Observed band size :100 kDa)

Related Product Information for anti-PEG10 antibody

Paternally expressed gene 10 (PEG10), located on human chromosome 7q21.3, is an oncogene implicated in the proliferation, apoptosis and metastasis of tumors. PEG10 has dual functions: accelerating cell growth by suppressing TGF-beta signaling and inhibiting cell motility and invasion by interfering with TGF-beta and BMP signaling via the AKT and p38 pathways, respectively. PEG10 protein is positively expressed in a variety of cancers with seemingly complex expression regulation mechanisms. PEG10 might be a molecular target for suppressing the aggressive phenotypes of chondrosarcoma cells. PEG10 expression is associated with advanced clinicopathological characteristics and can be used as a prognostic biomarker in patients with solid tumors.

References

Zhang M, et al. (2017) Peg10 is imperative for tgf-beta1-induced epithelial-mesenchymal transition in hepatocellular carcinoma. Oncol Rep 37 (1): 510-518.Xie T, et al. (2018) Peg10 as an oncogene: Expression regulatory mechanisms and role in tumor progression. Cancer Cell Int 18 112.Shapovalova M, et al. (2019) Peg10 promoter-driven expression of reporter genes enables molecular imaging of lethal prostate cancer. Cancer Res 79 (21): 5668-5680.