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Western Blot (WB) (Western blot analysis of Nestin antibody in K562 cell line lysates (35 ug/lane). NEST (arrow) was detected using the purified antibody.)

Rabbit Nestin Polyclonal Antibody | anti-NES antibody

Nestin Antibody

Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Affinity Chromatography
Synonyms
Nestin; Polyclonal Antibody; Nestin Antibody; Rabbit Polyclonal Nestin Antibody; Polyclonal Nestin antibody; anti-Nestin antibody; NES antibody; Nbla00170 antibody; FLJ21841 antibody; anti-NES antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Chromatography
Form/Format
Supplied in PBS buffer with 0.09% (W/V) Sodium Azide
Concentration
250 ug/ml (varies by lot)
Sequence Length
1621
Applicable Applications for anti-NES antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
ELISA: 1:1,000
FC/FACS: 1:10-50
WB: 1:100-500
Immunogen
Nestin antibody was raised in Rabbit using Nestin recombinant protein as the immunogen.
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Nestin antibody in K562 cell line lysates (35 ug/lane). NEST (arrow) was detected using the purified antibody.)

Western Blot (WB) (Western blot analysis of Nestin antibody in K562 cell line lysates (35 ug/lane). NEST (arrow) was detected using the purified antibody.)
Related Product Information for anti-NES antibody
Nestin may play a role in the trafficking and distribution of IF proteins and potentially other cellular factors to daughter cells during progenitor cell division.
Product Categories/Family for anti-NES antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nestin
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN

Uniprot Description

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; cell projection morphogenesis; brain development; negative regulation of neuron apoptosis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development

Similar Products

Product Notes

The NES nes (Catalog #AAA5311219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). ELISA: 1:1,000 FC/FACS: 1:10-50 WB: 1:100-500. Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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