Human RANkappa (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit | RANk clia kit

Human RANkappa (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit

Cat. Num. AAA2532431

• Gene Names: TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
• Reactivity: Human
• Synonyms: RANkappa (Receptor Activator of Nuclear Factor Kappa B); Human RANkappa (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit; RANK; TNFRSF11A; CD265; TRANCER; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; NFKB Activator; Human RANk (Receptor Activator of Nuclear Factor Kappa B) CLIA Kit; Human RANk; RANk clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant RANkappa. No significant cross-reactivity or interference between RANkappa and analogues was observed.
• Sequence Length: 317

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RANk clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of RANkappa concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to RANkappa. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RANkappa and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RANkappa, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of RANkappa. You can calculate the concentration of RANkappa in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18143619
• NCBI GeneID: 8600
• Molecular Weight: 30,523 Da
• NCBI Official Full Name: receptor activator of nuclear factor kappa B ligand 1
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 11
• NCBI Official Symbol: TNFSF11
• NCBI Official Synonym Symbols: ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TRANCE; hRANKL2
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: TNFSF11
• UniProt Synonym Gene Names: OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_HUMAN

NCBI Description

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: extracellular space; integral to plasma membrane; cytoplasm; extracellular region; plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoclast differentiation; cytokine and chemokine mediated signaling pathway; mammary gland epithelial cell proliferation; osteoclast differentiation; activation of JNK activity; activation of NF-kappaB transcription factor; positive regulation of corticotropin-releasing hormone secretion; positive regulation of homotypic cell-cell adhesion; calcium ion homeostasis; positive regulation of protein kinase B signaling cascade; positive regulation of MAP kinase activity; monocyte chemotaxis; organ morphogenesis; tumor necrosis factor-mediated signaling pathway; positive regulation of bone resorption; positive regulation of transcription factor activity; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell activation; protein homooligomerization; bone resorption

Disease: Osteopetrosis, Autosomal Recessive 2