Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nestin antibody. Counter stained with hematoxylin.)
Rabbit anti-Human Nestin Polyclonal Antibody | anti-NES antibody
Nestin Polyclonal Antibody
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
ProA affinity purified
Synonyms
Nestin; Polyclonal Antibody; Nestin Polyclonal Antibody; NES; ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST; anti-NES antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Nestin polyclonal antibody detects endogenous levels of Nestin protein.
Purity/Purification
ProA affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
1621
Applicable Applications for anti-NES antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
ICC: 1:50-1:200
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
recombinant protein
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nestin antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nestin antibody. Counter stained with hematoxylin.)
Related Product Information for anti-NES antibody
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or a-internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differen-tiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
NCBI and Uniprot Product Information
Uniprot Description
nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament
Molecular Function: intermediate filament binding; structural molecule activity
Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; cell projection morphogenesis; brain development; negative regulation of neuron apoptosis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
Similar Products
Product Notes
The Nestin nes (Catalog #AAA3013114) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nestin Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). ICC: 1:50-1:200 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the Nestin nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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