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Western Blot (WB) (Mouse Nek8 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)

Rabbit anti-Human, mouse Nek8 Polyclonal Antibody | anti-Nek8 antibody

Mouse Nek8 Antibody (C-term)

Gene Names
Nek8; jck; b2b1449Clo; 4632401F23Rik
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Nek8; Polyclonal Antibody; Mouse Nek8 Antibody (C-term); Serine/threonine-protein kinase Nek8; Never in mitosis A-related kinase 8; NimA-related protein kinase 8; Jck; anti-Nek8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Nek8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the C-terminal region of mouse Nek8.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
519-547
Sequence Length
698
Applicable Applications for anti-Nek8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Nek8 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)

Western Blot (WB) (Mouse Nek8 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Nek8 (arrow) using rabbit polyclonal Mouse Nek8 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek8 gene.)

Western Blot (WB) (Western blot analysis of Nek8 (arrow) using rabbit polyclonal Mouse Nek8 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek8 gene.)

Western Blot (WB)

(Mouse Nek8 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)

Western Blot (WB) (Mouse Nek8 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)
Related Product Information for anti-Nek8 antibody
This gene encodes a NIMA-related kinase. Members of this
serine/threonine protein kinase family are structurally-related to
NIMA (never in mitosis, gene A) which controls mitotic signaling in
Aspergillus nidulans.
Product Categories/Family for anti-Nek8 antibody
References
Hellman, N.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18499-18504(2010)
Ahmadie, R., et al. J. Nutr. 140(8):1438-1444(2010)
Natoli, T.A., et al. Nat. Med. 16(7):788-792(2010)
Shiba, D., et al. Cytoskeleton (Hoboken) 67(2):112-119(2010)
Sohara, E., et al. J. Am. Soc. Nephrol. 19(3):469-476(2008)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75265
NCBI Official Full Name
serine/threonine-protein kinase Nek8
NCBI Official Synonym Full Names
NIMA (never in mitosis gene a)-related expressed kinase 8
NCBI Official Symbol
Nek8
NCBI Official Synonym Symbols
jck; b2b1449Clo; 4632401F23Rik
NCBI Protein Information
serine/threonine-protein kinase Nek8
UniProt Protein Name
Serine/threonine-protein kinase Nek8
UniProt Gene Name
Nek8
UniProt Synonym Gene Names
Jck; NimA-related protein kinase 8
UniProt Entry Name
NEK8_MOUSE

NCBI Description

This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans. [provided by RefSeq, Jul 2008]

Uniprot Description

NEK8: a protein kinase related to the NIMA (never in mitosis, gene A) serine/threonine kinase of Aspergillus nidulans. May play a role in mitotic regulation.Required for renal tubular integrity.

Protein type: Protein kinase, tyrosine (non-receptor); EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; NEK family

Cellular Component: cytoskeleton; cell projection; cytoplasm; cilium

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: organ morphogenesis; mitotic cell cycle; phosphorylation; protein amino acid phosphorylation; microtubule-based process

Research Articles on Nek8

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Product Notes

The Nek8 nek8 (Catalog #AAA9208576) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 519-547. The Mouse Nek8 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nek8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Nek8 nek8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nek8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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