Rabbit anti-Human, Mouse Nek8 Polyclonal Antibody | anti-Nek8 antibody

Nek8, CT (Nek8, Jck, Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8) (FITC)

Cat. Num. AAA6324631

• Gene Names: Nek8; jck; b2b1449Clo; 4632401F23Rik
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Nek8; Polyclonal Antibody; CT (Nek8; Jck; Serine/threonine-protein kinase Nek8; Never in mitosis A-related kinase 8) (FITC); Never in mitosis A-related kinase 8; anti-Nek8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Nek8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Nek8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 518-547 amino acids from the C-terminal region of Mouse Nek8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Nek8 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Nek8 (arrow) using rabbit polyclonal Mouse Nek8 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek8 gene.)

Western Blot (WB)

(Mouse Nek8 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).)

Related Product Information for anti-Nek8 antibody

This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans.

Product Categories/Family for anti-Nek8 antibody

Antibodies; Protein Kinases

References

Hellman, N.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18499-18504(2010). Ahmadie, R., et al. J. Nutr. 140(8):1438-1444(2010). Natoli, T.A., et al. Nat. Med. 16(7):788-792(2010). Shiba, D., et al. Cytoskeleton (Hoboken) 67(2):112-119(2010). Sohara, E., et al. J. Am. Soc. Nephrol. 19(3):469-476(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 19263332
• NCBI GeneID: 140859
• NCBI Accession #: NP_543125.1
• NCBI GenBank Nucleotide #: NM_080849.3
• UniProt Accession #: Q91ZR4; Q3U498; Q9D685
• Molecular Weight: 75,265 Da
• NCBI Official Full Name: serine/threonine-protein kinase Nek8
• NCBI Official Synonym Full Names: NIMA (never in mitosis gene a)-related expressed kinase 8
• NCBI Official Symbol: Nek8
• NCBI Official Synonym Symbols: jck; b2b1449Clo; 4632401F23Rik
• NCBI Protein Information: serine/threonine-protein kinase Nek8; juvenile cystic kidney; nimA-related protein kinase 8; NIMA-related expressed kinase 8; never in mitosis A-related kinase 8
• UniProt Protein Name: Serine/threonine-protein kinase Nek8
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Nek8
• UniProt Synonym Gene Names: Jck; NimA-related protein kinase 8
• UniProt Entry Name: NEK8_MOUSE