Rabbit SHOX Polyclonal Antibody | anti-SHOX antibody

SHOX Polyclonal Antibody

Cat. Num. AAA3006489

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SHOX; Polyclonal Antibody; SHOX Polyclonal Antibody; Short stature homeobox protein; Pseudoautosomal homeobox-containing osteogenic protein; Short stature homeobox-containing protein; PHOG; anti-SHOX antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SHOX polyclonal antibody detects endogenous levels of SHOX protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 225

• Applicable Applications for anti-SHOX antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SHOX.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SHOX polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:sp2/0 whole cell lysate)

Related Product Information for anti-SHOX antibody

Homeodomain proteins (HP) are transcriptional regulators that coordinate the expression of genes involved in development, differentiation and cellular transformation. HPs are characterized by a conserved domain of 60 amino acid residues that recognize and bind a site in the regulatory region of the target gene. SHOX, a member of the bicoid subfamily of the paired homeobox family, controls fundamental aspects of growth and development and undergoes splicing resulting in two isoforms, SHOXA and SHOXB. SHOXA is expressed in skeletal muscle, placental, pancreas, heart and bone marrow fibroblast and, to a lesser extent, in kidney and lung. SHOXB is highly expressed in osteogenic cells, but not expressed in brain, kidney, liver or lung. Defects in the gene SHOXX cause Leri-Weill dyschondrosteosis (LWD) and Langer mesomelic dysplasia (LMD).

NCBI and Uniprot Product Information

• NCBI GI #: 6031203
• NCBI Accession #: NP_006874.1
• NCBI GenBank Nucleotide #: NP_006874.1
• UniProt Accession #: O15266
• Molecular Weight: ~ 29kDa
• NCBI Official Full Name: short stature homeobox protein isoform SHOXb
• UniProt Protein Name: Short stature homeobox protein
• Protein Family: Short stature homeobox protein
• UniProt Gene Name: SHOX
• UniProt Synonym Gene Names: PHOG
• UniProt Entry Name: SHOX_HUMAN

Uniprot Description

Function: Controls fundamental aspects of growth and development.

Subcellular location: Nucleus

By similarity.

Tissue specificity: SHOXA is expressed in skeletal muscle, placenta, pancreas, heart and bone marrow fibroblast and SHOXB is highly expressed in bone marrow fibroblast followed by kidney and skeletal muscle. SHOXB is not expressed in brain, kidney, liver and lung. Highly expressed in osteogenic cells.

Induction: By retinoic acid and phorbol-12-myristate 13-acetate (PMA).

Involvement in disease: Leri-Weill dyschondrosteosis (LWD) [MIM:127300]: Dominantly inherited skeletal dysplasia characterized by moderate short stature predominantly because of short mesomelic limb segments. It is often associated with the Madelung deformity of the wrist, comprising bowing of the radius and dorsal dislocation of the distal ulna.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.4 Ref.5 Ref.6Langer mesomelic dysplasia (LMD) [MIM:249700]: Autosomal recessive rare skeletal dysplasia characterized by severe short stature owing to shortening and maldevelopment of the mesomelic and rhizomelic segments of the limbs. Associated malformations are rarely reported and intellect is normal in all affected subjects reported to date.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5 Ref.7Short stature, idiopathic, X-linked (ISS) [MIM:300582]: A condition defined by a standing height more than 2 standard deviations below the mean (or below the 2.5 percentile) for sex and chronological age, compared with a well-nourished, genetically relevant population, in the absence of specific causative disorders.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1 Ref.5

Miscellaneous: The gene coding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.

Sequence similarities: Belongs to the paired homeobox family. Bicoid subfamily.Contains 1 homeobox DNA-binding domain.