Rabbit anti-Human, Mouse NEDD4 Polyclonal Antibody | anti-Nedd4 antibody

NEDD4, CT (NEDD4, KIAA0093, NEDD4-1, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, Neural precursor cell expressed developmentally down-regulated protein 4)

Cat. Num. AAA648777

• Gene Names: Nedd4; Nedd4a
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: NEDD4; Polyclonal Antibody; CT (NEDD4; KIAA0093; NEDD4-1; E3 ubiquitin-protein ligase NEDD4; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4); Anti -NEDD4; anti-Nedd4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Purified; Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Nedd4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:50-100; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: NEDD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 977~1007 amino acids from the C-terminal region of human NEDD4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Nedd4 antibody

Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.

Product Categories/Family for anti-Nedd4 antibody

Antibodies; Abs to Ubiquitin Ligases

NCBI and Uniprot Product Information

• NCBI GI #: 1293647
• NCBI GeneID: 25489
• UniProt Accession #: Q62940
• Molecular Weight: 102,395 Da
• NCBI Official Full Name: Nedd4
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• NCBI Official Symbol: Nedd4
• NCBI Official Synonym Symbols: Nedd4a
• NCBI Protein Information: E3 ubiquitin-protein ligase NEDD4; neural precursor cell expressed, developmentally down-regulated gene 4; neural precursor cell expressed, developmentally down-regulated gene 4A
• UniProt Protein Name: E3 ubiquitin-protein ligase NEDD4
• Protein Family: Nedd4 binding protein
• UniProt Gene Name: Nedd4
• UniProt Synonym Gene Names: Nedd4a
• UniProt Entry Name: NEDD4_RAT

NCBI Description

may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]

Uniprot Description

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Is involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2

By similarity. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding

By similarity. Ref.2

Enzyme regulation: Activated by NDFIP1- and NDFIP2-binding

By similarity.

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Interacts with UBE2D2. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1, and PRRG2

By similarity. Interacts (via C2 domain) with GRB10 (via SH2 domain)

By similarity. Binds SCNN1A, SCNN1B and SCNN1G. Interacts with ERBB4. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes

By similarity. Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4. Interacts (via WW3 domain) with TNK2; EGF promotes this interaction. Interacts (via WW3 domain) with FGFR1 (via C-terminus). Interacts with OTUD7B

By similarity. Interacts with ISG15

By similarity. Interacts (via WW domain) with RAPGEF2; this interaction leads to ubiquitination and degradation via the proteasome pathway

By similarity. Ref.1 Ref.2 Ref.3

Subcellular location: Cytoplasm. Cell membrane; Peripheral membrane protein

By similarity. Note: Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment

By similarity. May be recruited to exosomes by NDFIP1

By similarity. Ref.2

Tissue specificity: Ubiquitously expressed. Expression is highest in lung, kidney and brain. Ref.2

Developmental stage: Down-regulated after synapse formation. Ref.2

Domain: The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2

By similarity.

Post-translational modification: Auto-ubiquitinated

By similarity.

Miscellaneous: A cysteine residue is required for ubiquitin-thioester formation.

Sequence similarities: Contains 1 C2 domain.Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.Contains 3 WW domains.