Rabbit anti-Human, Mouse NEDD4 Polyclonal Antibody | anti-Nedd4 antibody
NEDD4, CT (NEDD4, KIAA0093, NEDD4-1, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, Neural precursor cell expressed developmentally down-regulated protein 4)
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Western Blot (WB)
(Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006]
Uniprot Description
Function: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Is involved in ubiquitination of ERBB4 intracellular domain E4ICD. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2
By similarity. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding
By similarity. Ref.2
Enzyme regulation: Activated by NDFIP1- and NDFIP2-binding
By similarity.
Pathway: Protein modification; protein ubiquitination.
Subunit structure: Interacts with UBE2D2. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with BEAN1, LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1, and PRRG2
By similarity. Interacts (via C2 domain) with GRB10 (via SH2 domain)
By similarity. Binds SCNN1A, SCNN1B and SCNN1G. Interacts with ERBB4. Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase and may induce its recruitment to exosomes
By similarity. Interacts with TNIK; the interaction is direct, allows the TNIK-dependent recruitment of RAP2A and its ubiquitination by NEDD4. Interacts (via WW3 domain) with TNK2; EGF promotes this interaction. Interacts (via WW3 domain) with FGFR1 (via C-terminus). Interacts with OTUD7B
By similarity. Interacts with ISG15
By similarity. Interacts (via WW domain) with RAPGEF2; this interaction leads to ubiquitination and degradation via the proteasome pathway
By similarity. Ref.1 Ref.2 Ref.3
Subcellular location: Cytoplasm. Cell membrane; Peripheral membrane protein
By similarity. Note: Recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, follows IGF1R internalization, remaining associated with early endosomes. Uncouples from IGF1R-containing endosomes before the sorting of the receptor to the lysosomal compartment
By similarity. May be recruited to exosomes by NDFIP1
By similarity. Ref.2
Tissue specificity: Ubiquitously expressed. Expression is highest in lung, kidney and brain. Ref.2
Developmental stage: Down-regulated after synapse formation. Ref.2
Domain: The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2
By similarity.
Post-translational modification: Auto-ubiquitinated
By similarity.
Miscellaneous: A cysteine residue is required for ubiquitin-thioester formation.
Sequence similarities: Contains 1 C2 domain.Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.Contains 3 WW domains.
