Rabbit anti-Human NEDD4 Polyclonal Antibody | anti-NEDD4 antibody

NEDD4 Polyclonal Antibody

Cat. Num. AAA3008437

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NEDD4; Polyclonal Antibody; NEDD4 Polyclonal Antibody; NEDD-4; KIAA0093; NEDD4-1; PIG53; anti-NEDD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NEDD4 polyclonal antibody detects endogenous levels of NEDD4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1319

• Applicable Applications for anti-NEDD4 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human NEDD4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using NEDD4 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate using NEDD4 antibody at dilution of 1:200 (40x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric using NEDD4 antibody at dilution of 1:200 (40x lens).)

Related Product Information for anti-NEDD4 antibody

NEDD4 is a 221 amino acid, Golgi-associated protein which contains three transmembrane domains in its carboxy-terminus and two PY motifs in its amino-terminus. NEDD4 may play a role in Golgi structure and function. It binds the WW domains of a number of NEDD4 family members called HECT-type E3 ubiquitin ligases. NEDD4 is strongly expressed in surviving neurons around an injury site, which suggests that ubiquitination may be a possible survival strategy and that NEDD4 may act as a neuroprotective protein. It may also have an effect on the function of Itch, another E3 ubiquitin ligase. Expression of NEDD4 and its association with Itch may be promoted by T cell activation.

NCBI and Uniprot Product Information

• NCBI GI #: 1812588811
• UniProt Accession #: P46934
• Molecular Weight: ~ 120kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase NEDD4
• UniProt Protein Name: E3 ubiquitin-protein ligase NEDD4
• Protein Family: Nedd4 binding protein
• UniProt Gene Name: NEDD4
• UniProt Synonym Gene Names: KIAA0093; NEDD4-1; NEDD-4
• UniProt Entry Name: NEDD4_HUMAN

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).