Rabbit anti-Human, Mouse NEDD4 Polyclonal Antibody | anti-NEDD4 antibody

NEDD4, CT (NEDD4, KIAA0093, NEDD4-1, E3 ubiquitin-protein ligase NEDD4, Cell proliferation-inducing gene 53 protein, Neural precursor cell expressed developmentally down-regulated protein 4) (APC)

Cat. Num. AAA6324497

• Gene Names: NEDD4; RPF1; NEDD4-1
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: NEDD4; Polyclonal Antibody; CT (NEDD4; KIAA0093; NEDD4-1; E3 ubiquitin-protein ligase NEDD4; Cell proliferation-inducing gene 53 protein; Neural precursor cell expressed developmentally down-regulated protein 4) (APC); Neural precursor cell expressed developmentally down-regulated protein 4; anti-NEDD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NEDD4. Species Crossreactivity: mouse
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NEDD4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: NEDD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 977~1007 amino acids from the C-terminal region of human NEDD4.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NEDD4 Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). NEDD4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(NEDD4 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NEDD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NEDD4 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NEDD4 antibody

Nedd4 is one of a group of mouse genes that show developmentally regulated expression in mouse embryonic brain. Nedd4 is expressed in various other embryonic tissues and persists in most adult tissues.

Product Categories/Family for anti-NEDD4 antibody

Antibodies; Ubiquitin Ligases

References

Lin, Q., et al. Mol. Cell. Biol. 30(6):1541-1554(2010). Crowther-Swanepoel, D., et al. Nat. Genet. 42(2):132-136(2010). Edwin, F., et al. J. Biol. Chem. 285(1):255-264(2010). Guo, Y.Y., et al. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 31(6):679-685(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 313104311
• NCBI GeneID: 4734
• UniProt Accession #: P46934; A1KY35; A6ND72; A7MD29; B4E2R7; B7ZM59; B7ZM60; B9EGN5; D6RF89
• Molecular Weight: 1319
• NCBI Official Full Name: E3 ubiquitin-protein ligase NEDD4
• NCBI Official Synonym Full Names: neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
• NCBI Official Symbol: NEDD4
• NCBI Official Synonym Symbols: RPF1; NEDD4-1
• NCBI Protein Information: E3 ubiquitin-protein ligase NEDD4; receptor-potentiating factor 1; cell proliferation-inducing gene 53 protein; neural precursor cell expressed developmentally down-regulated protein 4
• UniProt Protein Name: E3 ubiquitin-protein ligase NEDD4
• Protein Family: Nedd4 binding protein
• UniProt Gene Name: NEDD4
• UniProt Synonym Gene Names: KIAA0093; NEDD4-1; NEDD-4
• UniProt Entry Name: NEDD4_HUMAN

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates 'Lys-63' in target proteins (PubMed:23644597). Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes. Promotes ubiquitination of RAPGEF2. According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD. Involved in the budding of many viruses. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2. Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 (PubMed:25631046).