Protein Kinase N1 (PKN1) Recombinant Protein | PKN1 recombinant protein

Recombinant Protein Kinase N1 (PKN1)

Cat. Num. AAA2018769

• Gene Names: PKN1; DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Kinase N1 (PKN1); Recombinant Protein Kinase N1 (PKN1); PKN1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: Residues: Phe615~Phe874 (Accession # Q16512), with two N-terminal Tags, His-tag and T7-tag. The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- FKFLAV LGRGHFGKVL LSEFRPSGEL FAIKALKKGD IVARDEVESL MCEKRILAAV TSAGHPFLVN LFGCFQTPEH VCFVMEYSAG GDLMLHIHSD VFSEPRAIFY SACVVLGLQF LHEHKIVYRD LKLDNLLLDT EGYVKIADFG LCKEGMGYGD RTSTFCGTPE FLAPEVLTDT SYTRAVDWWG LGVLLYEMLV GESPFPGDDE EEVFDSIVND EVRYPRFLSA EAIGIMRRLL RRNPERRLGS SERDAEDVKK QPFF
• Sequence Length: 942

• Applicable Applications for PKN1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 25304069
• NCBI GeneID: 5585
• UniProt Accession #: Q16512; Q15143; Q504U4; Q8IUV5; Q9UD44; A8K7W5; B2R9R4; B3KVN3
• Molecular Weight: 32.9kDa
• NCBI Official Full Name: Protein kinase N1
• NCBI Official Synonym Full Names: protein kinase N1
• NCBI Official Symbol: PKN1
• NCBI Official Synonym Symbols: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
• NCBI Protein Information: serine/threonine-protein kinase N1; protein kinase C-like 1; protein kinase PKN-alpha; protein kinase C-like PKN; serine-threonine kinase N; protease-activated kinase 1; protein kinase C-related kinase 1; protein-kinase C-related kinase 1; serine-threonine protein kinase N; serine/threonine protein kinase N
• UniProt Protein Name: Serine/threonine-protein kinase N1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PKN1
• UniProt Synonym Gene Names: PAK1; PKN; PRK1; PRKCL1; PAK-1
• UniProt Entry Name: PKN1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

PKN1: an AGC kinase of the PKN family. A PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcriptional regulation. Regulates the cytoskeletal network by phosphorylating intermediate filament proteins such as VIM and NFH, NFL and NFM, inhibiting their polymerization. Phosphorylates Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of H3T11, a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3K9me by JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, impairing their import in the nucleus. Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids. Activated by CASP3 cleavage during apoptosis. Localization to endosomes is mediated via its interaction with RHOB. Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKN family

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; midbody; nucleus; endosome; cleavage furrow

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; protein kinase C binding; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; histone binding; histone deacetylase binding; Rac GTPase binding; GTP-Rho binding; chromatin binding; ATP binding; protein kinase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; hyperosmotic response; transcription, DNA-dependent; signal transduction; protein amino acid phosphorylation; activation of JNK activity