Heparan sulfate N-deacetylase/N-sulfotransferase 1 Recombinant Protein | NDST1 recombinant protein

Recombinant Human Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1

Cat. Num. AAA966196

• Gene Names: NDST1; HSST; NST1; MRT46
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Heparan sulfate N-deacetylase/N-sulfotransferase 1; Recombinant Human Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1; 2 domains: Heparan sulfate N-deacetylase 1 (EC:3.-.-.-); Heparan sulfate N-sulfotransferase 1 (EC:2.8.2.-); NDST1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 40-556aa; Partial of Isoform 2
• Sequence: GWKRGLEPSADAPEPDCGDPPPVAPSRLLPLKPVQAATPSRTDPLVLVFVESLYSQLGQEVVAILESSRFKYRTEIAPGKGDMPTLTDKGRGRFALIIYENILKYVNLDAWNRELLDKYCVAYGVGIIGFFKANENSLLSAQLKGFPLFLHSNLGLKDCSINPKSPLLYVTRPSEVEKGVLPGEDWTVFQSNHSTYEPVLLAKTRSSESIPHLGADAGLHAALHATVVQDLGLHDGIQRVLFGNNLNFWLHKLVFVDAVAFLTGKRLSLPLDRYILVDIDDIFVGKEGTRMKVEDVKALFDTQNELRAHIPNFTFNLGYSGKFFHTGTNAEDAGDDLLLSYVKEFWWFPHMWSHMQPHLFHNQSVLAEQMALNKKFAVEHGIPTDMGYAVAPHHSGVYPVHVQLYEAWKQVWSIRVTSTEEYPHLKPARYRRGFIHNGIMVLPRQTCGLFTHTIFYNEYPGGSSELDKIINGGELFLTVLLNPVSAPQPMAAGEKGLLHSLSAADTGFLEPGKGGEA
• Sequence Length: 556

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for NDST1 recombinant protein

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.

Product Categories/Family for NDST1 recombinant protein

Immunology

References

Cloning of the human heparan sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher Collins syndrome candidate region at 5q32-q33.1.Dixon J., Loftus S.K., Gladwin A.J., Scambler P.J., Wasmuth J.J., Dixon M.J.Genomics 26:239-244(1995) Localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase to the trans-Golgi network.Humphries D.E., Sullivan B.M., Aleixo M.D., Stow J.L.Biochem. J. 325:351-357(1997) Labell T.L., Milewicz D.J., Bonadio J., Edelhoff S., Disteche C.M., Byers P.H. A role of Lys614 in the sulfotransferase activity of human heparan sulfate N-deacetylase/N-sulfotransferase.Sueyoshi T., Kakuta Y., Pedersen L.C., Wall F.E., Pedersen L.G., Negishi M.FEBS Lett. 433:211-214(1998) Overexpression of different isoforms of glucosaminyl N-deacetylase/N-sulfotransferase results in distinct heparan sulfate N-sulfation patterns.Pikas D.S., Eriksson I., Kjellen L.Biochemistry 39:4552-4558(2000) Antibody-based assay for N-deacetylase activity of heparan sulfate/heparin N-deacetylase/N-sulfotransferase (NDST) novel characteristics of NDST-1 and -2.van den Born J., Pikas D.S., Pisa B.J., Eriksson I., Kjellen L., Berden J.H.M.Glycobiology 13:1-10(2003) Crystal structure of the sulfotransferase domain of human heparan sulfate N-deacetylase/N-sulfotransferase 1.Kakuta Y., Sueyoshi T., Negishi M., Pedersen L.C.J. Biol. Chem. 274:10673-10676(1999) NDST1 missense mutations in autosomal recessive intellectual disability.Reuter M.S., Musante L., Hu H., Diederich S., Sticht H., Ekici A.B., Uebe S., Wienker T.F., Bartsch O., Zechner U., Oppitz C., Keleman K., Jamra R.A., Najmabadi H., Schweiger S., Reis A., Kahrizi K.Am. J. Med. Genet. A 164A:2753-2763(2014)

NCBI and Uniprot Product Information

• NCBI GI #: 666637969
• NCBI GeneID: 3340
• NCBI Accession #: NP_001287992.1
• NCBI GenBank Nucleotide #: NM_001301063.1
• UniProt Accession #: P52848; Q96E57
• Molecular Weight: 73.5 kDa
• NCBI Official Full Name: bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 isoform 2
• NCBI Official Synonym Full Names: N-deacetylase/N-sulfotransferase 1
• NCBI Official Symbol: NDST1
• NCBI Official Synonym Symbols: HSST; NST1; MRT46
• NCBI Protein Information: bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
• UniProt Protein Name: Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
• UniProt Gene Name: NDST1
• UniProt Synonym Gene Names: HSST; HSST1; NDST-1; N-HSST 1; HSNST 1
• UniProt Entry Name: NDST1_HUMAN

NCBI Description

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis (PubMed:10758005, PubMed:12634318). Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response (PubMed:10758005, PubMed:12634318). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).