Rabbit GSK3alpha Polyclonal Antibody | anti-GSK3alpha antibody

GSK3alpha (Phospho-S21) Polyclonal Antibody

Cat. Num. AAA3004634

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GSK3alpha; Polyclonal Antibody; GSK3alpha (Phospho-S21) Polyclonal Antibody; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; anti-GSK3alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-GSK3alpha (S21) polyclonal antibody detects endogenous levels of GSK3alpha protein only when phosphorylated at Ser21.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 483

• Applicable Applications for anti-GSK3alpha antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human GSK3alpha around the phosphorylation site of Serine 21.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of p-GSK3alpha (S21) polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysate treated with UVLane2:Mouse liver tissue lysateLane3:Rat liver tissue lysateLane4:PC12 cell lysate treated with H2O2)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-GSK3a (S21) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of GSK3alpha (phospho-S21) pAb pAb at 1:1000 dilutionLane1:The Brain tissue lysate of MouseLane2:CT26 whole cell lysateLane3:C6 whole cell lysateLane4:L02 treated with PBS (1×PBS,PH7. 4) for 1 houre then added FBS (10%) for 15min whole cell lysateLane5:L02 treated with PBS (1×PBS,PH7. 4) for 1 houre then added FBS (10%) for 10min whole cell lysateLane6:L02 treated with PBS (1×PBS,PH7. 4) for 1 houre then added FBS (10%) for 5min whole cell lysateLane7:L02 whole cell lysate (40ug) Lane8:L02 treated with PBS (1×PBS,PH7. 4) for 1 houre whole cell lysateLane9:L02 treated with PBS (1×PBS,PH7. 4) for 1 houre then treated with DMEM (10%FBS) for 5min whole cell lysateLane10:L02 treated with PBS (1×PBS,PH7. 4) for 1 houre then treated with DMEM (10%FBS) for 10min whole cell lysateLane11:L02treated with PBS (1×PBS,PH7. 4) for 1 houre then treated with DMEM (10%FBS) for 15min whole cell lysate)

Related Product Information for anti-GSK3alpha antibody

Glycogen synthase kinase-3 alpha and beta (GSK-3alpha, beta) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3beta activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFalpha in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3alpha and GSK-3beta induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3alpha or Ser 9 of GSK-3beta and inactivates both forms

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NP_063937.2
• UniProt Accession #: P49840
• Molecular Weight: ~ 51kDa
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha
• UniProt Entry Name: GSK3A_HUMAN

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418

Protein type: EC 2.7.11.1; EC 2.7.11.26; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; GSK family; GSK subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: beta-catenin destruction complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; tau-protein kinase activity; ATP binding

Biological Process: glycogen metabolic process; proteasomal ubiquitin-dependent protein catabolic process; genetic imprinting; nerve growth factor receptor signaling pathway; negative regulation of insulin receptor signaling pathway; protein amino acid phosphorylation; negative regulation of transferase activity; positive regulation of cAMP biosynthetic process; negative regulation of glycogen biosynthetic process; epidermal growth factor receptor signaling pathway; nervous system development; negative regulation of TOR signaling pathway; cell migration; phosphoinositide-mediated signaling; Wnt receptor signaling pathway; fibroblast growth factor receptor signaling pathway; unfolded protein response; positive regulation of peptidyl-serine phosphorylation; negative regulation of glucose import; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; regulation of systemic arterial blood pressure; insulin receptor signaling pathway; positive regulation of protein catabolic process; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of heart contraction