Rabbit anti-Mouse Nck beta Polyclonal Antibody

Nck beta

Cat. Num. AAA614929

• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Nck beta; Polyclonal Antibody; Anti -Nck beta; anti-Nck beta antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Nck beta, Mr 47kD. Species Sequence Homology: human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-Nck beta antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 0.5-2ug/ml. Detects Nck beta in RIPA lysates of murine testes and spleen homogenates. Mouse testes homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with N0550-50 (2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.; Immunoprecipitation: 1:100. Immunoprecipitates; Nck beta from 1-2mg of mouse tissue homogenates.
• Immunogen: Synthetic peptide corresponding to aa175-190, EAPSFLSLRRGTALSN, from mouse Nck beta, conjugated to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of mouse testes homogenate using MBS614929 at 2ug/ml.)

Related Product Information for anti-Nck beta antibody

Nck is a highly conserved, oncogenic protein encoding one SH2 and three SH3 domains, the Src homology motifs found in nonreceptor tyrosine kinases, Ras GTPase-activating protein, phosphatidylinositol 3-kinase, and phospholipase C-gamma. Nck is a common target for the action of different surface receptors. Nck is widely expressed in various tissues and in cell lines from human, murine and rat origins. Nck is phosphorylated on tyrosine, serine and threonine residues in response to stimulation of EGF and PDGF in A431 and NIH 3T3 cells, respectively. Like other SH2-containing proteins, Nck is associated with tyrosine- autophosphorylated EGF or PDGF receptors via its SH2 domain. Overexpression of Nck leads to transformation of NIH 3T3 cells. The recombinant Nck precipitates endogenous WIP, a novel proline-rich protein that interacts with the Wiskott-Aldrich syndrome protein (WASP), from BJAB cell lysates. Nck binds through its second SH3 domain to WIP, and Nck binds to WIP at a site (aa321-415) that differs from the WASP-binding site (aa416-488). WIP has been shown to associate with the actin polymerization regulatory protein profilin and to induce actin polymerization and cytoskeletal reorganization in lymphoid cells.

Product Categories/Family for anti-Nck beta antibody

Antibodies; Abs to Proteins