Rabbit anti-Mouse Nck beta Polyclonal Antibody

Nck beta (MaxLight 750)

Cat. Num. AAA6489493

• Reactivity: Mouse
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Nck beta; Polyclonal Antibody; Nck beta (MaxLight 750); anti-Nck beta antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Nck beta, Mr 47kD. Species Sequence Homology: human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-Nck beta antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1:100. Immunoprecipitates; Applications are based on unconjugated antibody.; WB: 0.5-2ug/ml. Detects Nck beta in RIPA lysates of murine testes and spleen homogenates. Mouse testes homogenate was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
• Immunogen: Synthetic peptide corresponding to aa175-190, EAPSFLSLRRGTALSN, from mouse Nck beta, conjugated to KLH.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: Mouse testes homogenate probed with MBS614929 at 2ug/ml.)

Product Categories/Family for anti-Nck beta antibody

Antibodies; Proteins

References

1. Ling, P., et al., J. Biol. Chem. 276: 18, 908-18, 914 (2001).