PSMA/FOLH1 recombinant protein

Recombinant human PSMA/FOLH1 protein

Cat. Num. AAA201366

• Gene Names: FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: PSMA/FOLH1; Recombinant human PSMA/FOLH1 protein; Glutamate carboxypeptidase 2 isoform 1;Cell growth-inhibiting gene 27 protein; Folate hydrolase 1; Folylpoly-gamma-glutamate carboxypeptidase; Glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; N-acetylated-alpha-linked acidic dipeptidase I; Prostate-specific membrane antigen; Pteroylpoly-gamma-glutamate carboxypeptidase; Folh1; FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA; PSMA/FOLH1 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 20% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 44-750aa
• Sequence: KSSNEA TNITPKHNMK AFLDELKAEN IKKFLYNFTQ IPHLAGTEQN FQLAKQIQSQ WKEFGLDSVE LAHYDVLLSY PNKTHPNYIS IINEDGNEIF NTSLFEPPPP GYENVSDIVP PFSAFSPQGM PEGDLVYVNY ARTEDFFKLE RDMKINCSGK IVIARYGKVF RGNKVKNAQL AGAKGVILYS DPADYFAPGV KSYPDGWNLP GGGVQRGNIL NLNGAGDPLT PGYPANEYAY RRGIAEAVGL PSIPVHPIGY YDAQKLLEKM GGSAPPDSSW RGSLKVPYNV GPGFTGNFST QKVKMHIHST NEVTRIYNVI GTLRGAVEPD RYVILGGHRD SWVFGGIDPQ SGAAVVHEIV RSFGTLKKEG WRPRRTILFA SWDAEEFGLL GSTEWAEENS RLLQERGVAY INADSSIEGN YTLRVDCTPL MYSLVHNLTK ELKSPDEGFE GKSLYESWTK KSPSPEFSGM PRISKLGSGN DFEVFFQRLG IASGRARYTK NWETNKFSGY PLYHSVYETY ELVEKFYDPM FKYHLTVAQV RGGMVFELAN SIVLPFDCRD YAVVLRKYAD KIYSISMKHP QEMKTYSVSF DSLFSAVKNF TEIASKFSER LQDFDKSNPI VLRMMNDQLM FLERAFIDPL GLPDRPFYRH VIYAPSSHNK YAGESFPGIY DALFDIESKV DPSKAWGEVK RQIYVAAFTV QAAAETLSEV A

• Applicable Applications for PSMA/FOLH1 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for PSMA/FOLH1 recombinant protein

PSMA1/FOLH1, as known as glutamate carboxypeptidase 2 (GCPII), is a single pass type 2 membrane protein which belongs to the peptidase M28 family. It is a zinc metalloenzyme that resides in membranes and catalyzes the hydrolysis of N-acetylaspartylglutamate (NAAG) to glutamate and N-acetylaspartate (NAA). This protein is most highly expressed in prostate epithelium. It is detected in urinary bladder, kidney, testis, ovary, live, stomach, small intestine colon, and the capillary endothelium of a variety of tumors. Thus, it shows a promising role in directed imaging and therapy of recurrent of metastatic disease. Recombinant human PSMA1/FOLH1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for PSMA/FOLH1 recombinant protein

Signal Transduction; Metabolism; Proteins

References

Rahn KA., et al, (2012) Proc. Natl. Acad. Sci. U.S.A. 109:20101-20106. Schaevitz LR., et al, (2012) Dev Neurobiol 72:891-905.

NCBI and Uniprot Product Information

• NCBI GI #: 548615
• NCBI GeneID: 2346
• NCBI Accession #: NP_004467
• NCBI GenBank Nucleotide #: NM_004476
• UniProt Accession #: Q04609
• Molecular Weight: 84kDa
• NCBI Official Full Name: Glutamate carboxypeptidase 2
• NCBI Official Synonym Full Names: folate hydrolase 1
• NCBI Official Symbol: FOLH1
• NCBI Official Synonym Symbols: PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
• NCBI Protein Information: glutamate carboxypeptidase 2
• UniProt Protein Name: Glutamate carboxypeptidase 2
• UniProt Gene Name: FOLH1
• UniProt Synonym Gene Names: FOLH; NAALAD1; PSM; PSMA; FGCP; GCPII; mGCP; NAALADase I; PSM; PSMA
• UniProt Entry Name: FOLH1_HUMAN

NCBI Description

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]

Uniprot Description

FOLH1: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.4.17.21; Protease

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: membrane; integral to plasma membrane; cytoplasm

Molecular Function: peptidase activity; dipeptidase activity; metallopeptidase activity; carboxypeptidase activity; metal ion binding

Biological Process: folic acid and derivative metabolic process; proteolysis