Rabbit Sprouty 2 Polyclonal Antibody | anti-SPRY2 antibody

Sprouty 2 (Sprouty-2, Sprouty Homolog 2 (Drosophila), Spry 2, Spry2, Spry-2, hSPRY2, MGC23039)

Cat. Num. AAA616727

• Gene Names: SPRY2; hSPRY2; MGC23039
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Sprouty 2; Polyclonal Antibody; Sprouty 2 (Sprouty-2; Sprouty Homolog 2 (Drosophila); Spry 2; Spry2; Spry-2; hSPRY2; MGC23039); Anti -Sprouty 2 (Sprouty-2; anti-SPRY2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Sprouty2, Mr ~35-40kD. A non-specific protein was also detected, Mr ~70kD. Species Crossreactivity: Human and mouse. Crossreactivity with rat predicted, based on sequence homology.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

• Applicable Applications for anti-SPRY2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.1-1ug/ml detects Sprouty2 in RIPA lysates from HeLa and 3T3/A31 cells.
• Immunogen: KLH-conjugated, synthetic peptide corresponding to aa26-39 (C-GEPDPRDALTQQVH) of human Sprouty2, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat and mouse.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sprouty2 (0.2ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Sprouty2 ( 35-40kD).)

Related Product Information for anti-SPRY2 antibody

Sprouty is a suppressor of EGF and FGF signaling that appears to have a universal inhibitory role on tyrosine kinase signaling. Evidence suggests that it does this by binding adapter proteins and GAP, thus inactivating Ras. Sprouty expression is induced by the same pathways that it down-regulates, suggesting it plays a role in an auto-feedback loop. Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty-2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C-terminus of the protein. Significant sequence divergence occurs at the N-terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Product Categories/Family for anti-SPRY2 antibody

Antibodies; Abs to Sprouty

NCBI and Uniprot Product Information

• NCBI GI #: 2809400
• NCBI GeneID: 10253
• UniProt Accession #: O43597; Q5T6Z7; Q7Z783
• Molecular Weight: 34,688 Da
• NCBI Official Full Name: Sprouty 2
• NCBI Official Synonym Full Names: sprouty homolog 2 (Drosophila)
• NCBI Official Symbol: SPRY2
• NCBI Official Synonym Symbols: hSPRY2; MGC23039
• NCBI Protein Information: protein sprouty homolog 2; spry-2; sprouty 2; OTTHUMP00000018543
• UniProt Protein Name: Protein sprouty homolog 2
• Protein Family: Protein sprouty
• UniProt Gene Name: SPRY2
• UniProt Entry Name: SPY2_HUMAN

NCBI Description

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq]

Uniprot Description

SPRY2: a feedback inhibitor of growth factor receptor signaling. Belongs to the sprouty family. May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold; Inhibitor

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: microtubule cytoskeleton; microtubule; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase inhibitor activity; protein serine/threonine kinase activator activity; protein binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; cell fate commitment; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of nerve growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; negative regulation of cell proliferation; positive regulation of protein kinase B signaling cascade; sensory perception of sound; establishment of mitotic spindle orientation; negative regulation of Ras protein signal transduction; negative regulation of apoptosis